Novel salts and polymorphs of scy-078

ABSTRACT

SCY-078 is a glucan synthase inhibitor with antimicrobial activity. Novel salts and polymorph forms of SCY-078 are disclosed herein. The disclosure also relates to pharmaceutical compositions, methods of use, and methods of preparing the novel salts and polymorphs of SCY-078.

This application claims the benefit of priority to International Application No. PCT/CN2015/070967, filed Jan. 19, 2015, the contents of which are incorporated herein by reference.

SCY-078 (or “compound 1”) is a glucan synthase inhibitor useful as an antifungal compound. SCY-078 is useful for treating, among other things, Invasive Candidiasis and Invasive Aspergillosis. Other antimicrobial utilities of SCY-078 are disclosed, for example, in U.S. Pat. No. 8,188,085, the relevant portions of which are incorporated herein by reference. SCY-078 has the following chemical structure:

The present disclosure is directed to, among other things, pharmaceutically acceptable salts of SCY-078 and polymorphs of those salts. In another embodiment, the salts and polymorphs thereof exhibit certain kinetic solubilities. Higher kinetic solubilities can be importance in formulations, such as intravenous formulations. In yet another embodiment, the salts and polymorphs thereof exhibit certain hygroscopicities. Hygroscopity has been found to play an important role in the preparation of solid dosage forms and the selection of excipients. The present disclosure is further directed to, among other things, methods of preparing pharmaceutically acceptable salts of SCY-078 and polymorphs thereof.

The present disclosure is additionally directed to, among other things, pharmaceutical compositions comprising pharmaceutically acceptable salts of SCY-078 and polymorphs thereof. In another embodiment, the disclosure relates to methods of preparing pharmaceutical compositions comprising pharmaceutically acceptable salts of SCY-078 and polymorphs thereof suitable for injection or intravenous administration. In yet another embodiment, the present disclosure relates to method of treating fungal infections by administering pharmaceutically acceptable salts of SCY-078 and polymorphs thereof.

BRIEF DESCRIPTION OF DRAWINGS

FIG. 1 is an X-ray Powder Diffraction “XRPD” pattern of SCY-078 phosphate from Example 1.

FIG. 2 is a DSC curve and a TGA curve of SCY-078 phosphate from Example 1.

FIG. 3 is an XRPD of SCY-078 crystalline freebase (MeOH desolvate), batch 1 from Example 2.

FIG. 4 is a DSC curve and a TGA curve of SCY-078 crystalline freebase (MeOH desolvate), batch 1 from Example 2.

FIG. 5 is an XRPD of SCY-078 crystalline freebase (MeOH desolvate), batch 2 from Example 2.

FIG. 6 is a DSC curve and a TGA curve of SCY-078 amorphous freebase from Example 3.

FIG. 7 is an XRPD of SCY-078 HCl Type A from Example 7.

FIG. 8 is a DSC curve and a TGA curve of SCY-078 HCl Type A from Example 7.

FIG. 9 is an XRPD of SCY-078 citrate Type A from Example 8.

FIG. 10 is a DSC curve and a TGA curve of SCY-078 citrate Type A from Example 8.

FIG. 11 is an XRPD of SCY-078 hippurate Type A from Example 9.

FIG. 12 is a DSC curve and a TGA curve of SCY-078 hippurate Type A from Example 9.

FIG. 13 is an XRPD of SCY-078 hippurate Type B from Example 10 overlaid on an XRPD of SCY-078 hippurate Type A from Example 9.

FIG. 14 is a DSC curve and a TGA curve of SCY-078 hippurate Type B from Example 10.

FIG. 15 is an XRPD of SCY-078 hippurate Type C from Example 11 overlaid on the XPRDs of SCY-078 hippurate Type A, hippurate Type B, and hippurate Type B heated to 175° C.

FIG. 16 is a cycle DSC curve of SCY-078 hippurate Type B and SCY-078 hippurate Type C from Example 11.

FIG. 17 is an XRPD of SCY-078 fumarate Type A from Example 12.

FIG. 18 is a DSC curve and a TGA curve of SCY-078 fumarate Type A from Example 12.

FIG. 19 is an XRPD of SCY-078 fumarate Type B from Example 13.

FIG. 20 is a DSC curve and a TGA curve of SCY-078 fumarate Type B from Example 13.

FIG. 21 is an XRPD of SCY-078 glycolate Type A from Example 14.

FIG. 22 is a DSC curve and a TGA curve of SCY-078 glycolate Type A from Example 14.

FIG. 23 is an XRPD of SCY-078 mesylate Type A from Example 15.

FIG. 24 is a DSC curve and a TGA curve of SCY-078 mesylate Type A from Example 15.

FIG. 25 is an XRPD of SCY-078 calcium Type A from Example 16.

FIG. 26 is a DSC curve and a TGA curve of SCY-078 calcium Type A from Example 16.

FIG. 27 is an XRPD of scaled-up SCY-078 hippurate Type B from Example 17.

FIG. 28 is a DSC curve and a TGA curve of scaled-up SCY-078 hippurate Type B from Example 17.

FIG. 29 is a DVS isotherm plot of scaled-up SCY-078 hippurate Type B from Example 17.

FIG. 30 is an XRPD of scaled-up SCY-078 hippurate Type B before DVS, after DVS, and compared to hippurate Type A from Example 17.

FIG. 31 is an XRPD of scaled-up SCY-078 fumarate Type A from Example 18.

FIG. 32 is a DSC curve and a TGA curve of scaled-up SCY-078 fumarate Type A from Example 18.

FIG. 33 is a DVS isotherm plot of scaled-up SCY-078 fumarate Type A from Example 18.

FIG. 34 is an XRPD of scaled-up SCY-078 mesylate Type A from Example 19.

FIG. 35 is a DSC curve and a TGA curve of scaled-up SCY-078 mesylate Type A from Example 19.

FIG. 36 is a DVS isotherm plot of scaled-up SCY-078 mesylate Type A from Example 19.

FIG. 37 is an XRPD of scaled-up SCY-078 phosphate Type A from Example 20.

FIG. 38 is a DSC curve and a TGA curve of scaled-up SCY-078 phosphate Type A from Example 20.

FIG. 39 is a DVS isotherm plot of scaled-up SCY-078 phosphate Type A from Example 20.

FIG. 40 is an XRPD of scaled-up SCY-078 citrate Type A from Example 21.

FIG. 41 is a DSC curve and a TGA curve of scaled-up SCY-078 citrate Type A from Example 21.

FIG. 42 is a DVS isotherm plot of scaled-up SCY-078 citrate Type A from Example 21.

FIG. 43 is an XRPD of scaled-up SCY-078 citrate Type A from Example 26.

FIG. 44 is a DSC curve and a TGA curve of scaled-up SCY-078 citrate Type A from Example 26.

FIG. 45 is an XRPD of SCY-078 citrate Type A from Example 38.

FIG. 46 is a DSC curve and a TGA curve of SCY-078 citrate Type A from Example 38.

FIG. 47 is a DVS isotherm plot of SCY-078 citrate Type A from Example 38.

FIG. 48 is an XRPD of SCY-078 citrate Type A after process development from Example 38.

FIG. 49 is a DSC curve and a TGA curve of SCY-078 citrate Type A after process development from Example 38.

FIG. 50 is a DVS isotherm plot of SCY-078 citrate Type A after process development from Example 38.

FIG. 51 is an XRPD of SCY-078 citrate Type B from Example 39.

FIG. 52 is a DSC curve and a TGA curve of SCY-078 citrate Type B from Example 39.

FIG. 53 is an XRPD of SCY-078 citrate Type E from Example 40.

FIG. 54 is an XRPD of SCY-078 citrate Type E from Example 40 before and after storage.

FIG. 55 is an XRPD of SCY-078 citrate Type F from Example 41.

FIG. 56 is a DSC curve and a TGA curve of SCY-078 citrate Type F from Example 41.

FIG. 57 is an XRPD of SCY-078 citrate Type M from Example 42.

FIG. 58 is a DSC curve and a TGA curve of SCY-078 citrate Type M from Example 42.

FIG. 59 is a DVS isotherm plot of SCY-078 citrate Type M from Example 42.

FIG. 60 is an XRPD of SCY-078 citrate Type M from Example 42 at variable temperatures.

FIG. 61 is an XRPD of SCY-078 citrate Type N from Example 43.

FIG. 62 is an XRPD of SCY-078 citrate Type N from Example 43 before and after drying.

FIG. 63 is an XRPD of SCY-078 citrate Type 0 from Example 44.

FIG. 64 is an XRPD of SCY-078 citrate Type Q from Example 45.

FIG. 65 is an XRPD of SCY-078 citrate Type Q from Example 45 before and after drying.

FIG. 66 is an XRPD of SCY-078 citrate Type R from Example 46.

FIG. 67 is an XRPD of SCY-078 citrate Type R from Example 46 before and after drying.

FIG. 68 is an XRPD of SCY-078 citrate Type S from Example 47.

FIG. 69 is a DSC curve and a TGA curve of SCY-078 citrate Type S from Example 47.

FIG. 70 is a DVS isotherm plot of SCY-078 citrate Type S from Example 47.

FIG. 71 is an XRPD of SCY-078 citrate Type S from Example 47 at variable temperatures.

FIG. 72 shows XRPD patterns of SCY-078 citrate Types C, I, J, and P from Example 48.

FIG. 73 shows XRPD patterns of slurry conversion of SCY-078 citrate Type A or Type B to Type N from Example 50.

FIG. 74 shows XRPD patterns of SCY-078 citrate Type Q after drying from Example 50.

FIG. 75 shows XRPD patterns of SCY-078 citrate Types R, E, and M from Example 51.

FIG. 76 shows XRPD patterns of SCY-078 citrate Type M slurry in acetone from Example 51.

FIG. 77 shows XRPD patterns of SCY-078 citrate Type M slurry in acetonitrile from Example 51.

FIG. 78 shows XRPD patterns of SCY-078 citrate Type M slurry in MeOH/IPAc from Example 51.

FIG. 79 shows XRPD patterns of SCY-078 citrate Type M slurry in heptane from Example 51.

FIG. 80 shows XRPD patterns of SCY-078 citrate Type M after stability testing from Example 52.

FIG. 81 shows XRPD patterns of SCY-078 citrate Type S after stability testing from Example 52.

FIG. 82 is an XRPD of SCY-078 citrate Type A from Example 53.

FIG. 83 is a DSC curve and a TGA curve of SCY-078 citrate Type A from Example 53.

FIG. 84 is a DSC curve and a TGA curve of SCY-078 citrate Type A from Example 53.

FIG. 85 is an XRPD of SCY-078 Trifluoroacetate Type A from Example 54.

FIG. 86 is a DSC curve and a TGA curve of SCY-078 Trifluoroacetate Type A from Example 54.

FIG. 87 is an XRPD of SCY-078 Trifluoroacetate Type A before and after storage from Example 54.

FIG. 88 is an XRPD of SCY-078 Trifluoroacetate Type B from Example 55.

FIG. 89 is a DSC curve and a TGA curve of SCY-078 Trifluoroacetate Type B from Example 55.

FIG. 90 is a DVS curve of SCY-078 Trifluoroacetate Type B from Example 55.

FIG. 91 is an XRPD overlay of SCY-078 Trifluoroacetate Type B before and after DVS from Example 55.

FIG. 92 is an XRPD overlay of SCY-078 Trifluoroacetate Type B at varying relative humidity from Example 55.

FIG. 93 is a VT-XRPD overlay of SCY-078 Trifluoroacetate Type A from Example 55.

FIG. 94 is an XRPD of SCY-078 HCl Type I from Example 56.

FIG. 95 is a DSC curve and a TGA curves of SCY-078 HCl Type I from Example 56.

FIG. 96 is a DVS curve of SCY-078 HCl Type I from Example 56.

FIG. 97 is an XRPD overlay of SCY-078 HCl Type I before and after DVS from Example 56.

FIG. 98 is an XRPD of SCY-078 HCl Type II from Example 57.

FIG. 99 is a DSC curve and TGA curve of SCY-078 HCl Type II from Example 57.

FIG. 100 shows the kinetic solubility curves of SCY-078 Trifluoroacetate Types A and B and SCY-078 HCl Type I in SGF from Example 58.

FIG. 101 is an XRPD overlay of SCY-078 Trifluoroacetate Type A in SGF from Example 58.

FIG. 102 is an XRPD overlay of SCY-078 Trifluoroacetate Type B in SGF from Example 58.

FIG. 103 is an XRPD overlay of SCY-078 HCl Type I in SGF from Example 58.

FIG. 104 shows the kinetic solubility curves of SCY-078 Trifluoroacetate Types A and B and SCY-078 HCl Type I from Example 59.

FIG. 105 is an XRPD overlay of SCY-078 Trifluoroacetate Type A from Example 59.

FIG. 106 is an XRPD overlay of SCY-078 Trifluoroacetate Type B from Example 59.

FIG. 107 is an XRPD overlay of SCY-078 HCl Type I from Example 59.

FIG. 108 shows the kinetic solubility curves of SCY-078 Trifluoroacetate Types A and B and SCY-078 HCl Type I from Example 60.

FIG. 109 is an XRPD overlay of SCY-078 Trifluoroacetate Type A from Example 60.

FIG. 110 is an XRPD overlay of SCY-078 Trifluoroacetate Type B from Example 60.

FIG. 111 is an XRPD overlay of SCY-078 HCl Type I from Example 60.

FIG. 112 shows the kinetic solubility curves of SCY-078 Trifluoroacetate Types A and B and SCY-078 HCl Type I from Example 61.

FIG. 113 is an XRPD overlay of SCY-078 Trifluoroacetate Type A from Example 61.

FIG. 114 is an XRPD overlay of SCY-078 Trifluoroacetate Type B from Example 61.

FIG. 115 is an XRPD overlay of SCY-078 HCl Type I from Example 61.

FIG. 116 shows the kinetic solubility curves of SCY-078 Trifluoroacetate Types A and B and SCY-078 HCl Type I from Example 62.

FIG. 117 is an XRPD overlay of SCY-078 Trifluoroacetate Type A from Example 62.

FIG. 118 is an XRPD overlay of SCY-078 Trifluoroacetate Type B from Example 62.

FIG. 119 is an XRPD overlay of SCY-078 HCl Type I from Example 62.

FIG. 120 is an XRPD overlay of SCY-078 Trifluoroacetate Type A under 25° C./60% RH from Example 63.

FIG. 121 is an XRPD overlay of SCY-078 Trifluoroacetate Type A under 40° C./75% RH from Example 63.

FIG. 122 is an XRPD overlay of SCY-078 Trifluoroacetate Type A under 60° C. from Example 63.

FIG. 123 is an XRPD overlay of SCY-078 Trifluoroacetate Type B under 25° C./60% RH from Example 63.

FIG. 124 is an XRPD overlay of SCY-078 Trifluoroacetate Type B under 40° C./75% RH from Example 63.

FIG. 125 is an XRPD overlay of SCY-078 Trifluoroacetate Type B under 60° C. from Example 63.

FIG. 126 is an XRPD overlay of SCY-078 HCl Type I under 25° C./60% RH from Example 63.

FIG. 127 is an XRPD overlay of SCY-078 HCl Type I under 40° C./75% RH from Example 63.

FIG. 128 is an XRPD overlay of SCY-078 HCl Type I under 60° C. from Example 63.

DETAILED DESCRIPTION

All numbers used herein, including those in the examples and claims, should be understood as being modified by the term “about” unless otherwise stated, such as with a specified precision. Unless expressly stated to the contrary, all ranges cited herein are inclusive.

As used herein, the singular forms “a,” “an,” and “the” include plural reference unless the context dictates otherwise.

The terms “SCY-078” and “compound 1” refer to the compound shown below, and refer to the freebase form unless otherwise indicated. Another name for SCY-078 is (1S,4aR,6aS,7R,8R,10aR,10bR,12aR,14R,15R)-15-[[(2R)-2-amino-2,3,3-trimethylbutyl]oxy]-8-[(1R)-1,2-dimethylpropyl]-1445-(4-pyridinyl)-1H-1,2,4-triazol-1-yl]-1,6,6a,7,8,9,10,10a,10b,11,12,12a-dodecahydro-1,6a,8,10a-tetramethyl-4H-4a-propano-2H -phenanthro[1,2-c]pyran-7-carboxylic acid.

The term “HCl” refers to hydrochloric acid. The term “Ca” refers to calcium.

The terms “pharmaceutically acceptable salt” and the like should be understood to include, but not limited to, citrate salts, hippurate salts, fumarate salts, glycolate salts, mesylate salts, and calcium salts.

As used here, phrases such as “SCY-078 salt,” “SCY-078 salts,” “salt of SCY-078,” “salts of SCY-078,” “pharmaceutically acceptable salt of SCY-078,” and “pharmaceutically acceptable salts thereof” should be understood to be salts in various forms, for example, the polymorphs disclosed herein. In addition, as used here, phrases such as “SCY-078 phosphate,” “SCY-078 citrate,” “SCY-078 hippurate,” “SCY-078 glycolate,” “SCY-078 mesylate,” “SCY-078 fumarate,” and “SCY-078 calcium” should be understood to be salts in various forms, for example, the polymorphs disclosed herein.

The term “solvent” and the like refer to any appropriate aqueous or organic solvent. Solvents include, but are not limited to, methanol, acetic acid, tetrahydrofuran, 2 methyl-tetrahydrofuran, 1,4-dioxane, n-methyl-2-pyrrolidone, dimethyl sulfoxide, dimethylacetamide, isopropyl alcohol, acetonitrile, acetone, ethyl acetate, water and mixtures thereof.

The term “pharmaceutically acceptable carrier” and the like refer to an ingredient that is compatible with SCY-078 and is not harmful to a patient's health. Pharmaceutically acceptable carriers include, but are not limited to, one or more of the following: aqueous vehicles and solvents, such as water, saline solutions, and alcohols; buffers; surface active agents; dispersing agents; inert diluents; preservatives; suspending agents; emulsifying agents; demulcents; thickening agents; emulsifying agents; antioxidants; and stabilizing agents. Other additional ingredients that may be included in the pharmaceutical compositions of the disclosure are generally known in the art and may be described, for example, in Remington's Pharmaceutical Sciences, Mack Publishing Co., Easton, Pa., which is incorporated by reference herein.

The term “injection” and the like refer to the insertion of a composition into the body by syringe, hollow needle, or the like. The term “injection” and the like include, but are not limited to, intravenous injections, including those entailing administering using an IV bag containing a diluent.

The term “effective amount” refers to an amount of the active ingredient that, when administered to a subject, alleviates at least some of the symptoms or stops the progression of the identified disease or condition. The terms “disease” or “condition” include, but are not limited to, infections such as fungal infections. Exemplary dosage amounts can be found, for example, in U.S. Pat. No. 8,188,085, the relevant portions of which are incorporated herein by reference.

The term “Å” refers to angstroms. Terms such as “2θ” or “2 Th.” refer to degrees 2 theta.

The XRPD peaks recited herein should be understood to reflect a precision of ±0.2 for the 2 theta peaks, and the equivalent precision for d-spacings as per Bragg's law. The present disclosure also fully incorporates section 941 of the United States Pharmacopeia. The National Formulary from 2014 (USP 37/NF 32, volume 1) relating to characterization of crystalline and partially crystalline solids by X-ray Powder Diffraction.

The present disclosure relates to, among other things, pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, glycolate, mesylate, fumarate, and calcium. In one embodiment, the salt is selected from citrate, hippurate, mesylate, and fumarate. In a further embodiment, the SCY-078 salt is selected from SCY-078 hippurate Type A, SCY-078 hippurate Type B, and SCY-078 hippurate Type C. In another embodiment, the SCY-078 salt is selected from SCY-078 fumarate Type A and SCY-078 fumarate Type B. In yet another embodiment, the salt is a SCY-078 citrate salt. In yet a further embodiment, the salt is SCY-078 citrate Type A.

The present disclosure further relates to pharmaceutically acceptable salts of SCY-078 that have a chemical purity of at least 90%. In another embodiment, pharmaceutically acceptable salts of SCY-078 have a chemical purity of at least 95%. In a further embodiment, pharmaceutically acceptable salts of SCY-078 have a chemical purity of at least 98%. In yet another embodiment, pharmaceutically acceptable salts of SCY-078 have a chemical purity of at least 99%. In still another embodiment, the present disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a chemical purity of at least 90%, at least 95%, at least 98%, or at least 99%.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078 that have a kinetic solubility of at least 2 mg/mL at 4 hours in dextrose buffer at pH 5.5. In one embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 4 mg/mL at 4 hours in dextrose buffer at pH 5.5. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 2 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 4 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5. In still another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility greater than SCY-078 (as a freebase) at 4 hours in dextrose buffer at pH 5.5.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078 that have a kinetic solubility of at least 2 mg/mL at 24 hours in dextrose buffer at pH 5.5. In one embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 4 mg/mL at 24 hours in dextrose buffer at pH 5.5. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 8 mg/mL at 24 hours in dextrose buffer at pH 5.5. In a further embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 2 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 4 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5. In still another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 8 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 2 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5. In one embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 4 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5.

In yet another embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 2 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5. In still another embodiment, the disclosure also relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 4 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5. In another embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 8 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078 that have a kinetic solubility of at least 2 mg/mL at 4 hours in phosphate buffer at pH 6.0. In one embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 4 mg/mL at 4 hours in phosphate buffer at pH 6.0. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 2 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 4 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0.

In still another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 4.5 mg/mL at 24 hours in phosphate buffer at pH 6.0. In one embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 7 mg/mL at 24 hours in phosphate buffer at pH 6.0. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 4.5 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 7 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 1 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0. In one embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 4 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0.

In another embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 4 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0. In yet another embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 7 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078 that have a kinetic solubility of at least 16 mg/mL at 1 hour in SGF media. In one embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 17 mg/mL at 1 hour in SGF media. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 18 mg/mL at 1 hour in SGF media. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 20 mg/mL at 1 hour in SGF media.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078 that have a kinetic solubility of from 17 mg/mL to 21 mg/mL at 1 hour in SGF media. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 18 mg/mL to 21 mg/mL at 1 hour in SGF media. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 20 mg/mL to 21 mg/mL at 1 hour in SGF media.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 12 mg/mL to 21 mg/mL at 1 hour in SGF media. In one embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 13 mg/mL to 21 mg/mL at 1 hour in SGF media. In another embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 18 mg/mL to 21 mg/mL at 1 hour in SGF media. In yet another embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 20 mg/mL to 21 mg/mL at 1 hour in SGF media.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078 that have a kinetic solubility of at least 17 mg/mL at 24 hours in FaSSIF media. In one embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of at least 22 mg/mL at 24 hours in FaSSIF media. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 17 mg/mL to 22 mg/mL at 24 hours in FaSSIF media. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a kinetic solubility of from 21 mg/mL to 22 mg/mL at 24 hours in FaSSIF media.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 17 mg/mL to 22 mg/mL at 24 hours in FaSSIF media. In one embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a kinetic solubility of from 21 mg/mL to 22 mg/mL at 24 hours in FaSSIF media.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078 having any of the disclosed kinetic solubilities and having a water sorption of not greater than 7% at 25° C. and 80% relative humidity as determined by DVS. In one embodiment, the pharmaceutically acceptable salts of SCY-078 have a water sorption of from 2% to 7% at 25° C. and 80% relative humidity as determined by DVS. In another embodiment, the pharmaceutically acceptable salts of SCY-078 have a water sorption of from 3% to 7% at 25° C. and 80% relative humidity as determined by DVS. In yet another embodiment, the pharmaceutically acceptable salts of SCY-078 have a water sorption of from 6% to 7% at 25° C. and 80% relative humidity as determined by DVS.

The present disclosure additionally relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a water sorption of from 2% to 7% at 25° C. and 80% relative humidity as determined by DVS. In one embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a water sorption of from 3% to 7% at 25° C. and 80% relative humidity as determined by DVS. In one embodiment, the disclosure relates to pharmaceutically acceptable salts of SCY-078, wherein the salt is selected from citrate, hippurate, mesylate, and fumarate, and wherein the salt has a water sorption of from 6% to 7% at 25° C. and 80% relative humidity as determined by DVS.

The present disclosure further relates to hippurate salts of SCY-078, such as SCY-078 hippurate Type A, SCY-078 hippurate Type B, and SCY-078 hippurate Type C. In one embodiment, the SCY-078 hippurate Type A has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE A FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 6.276484 353.472400 0.102336 14.08226 12.90 7.023845 139.782300 0.307008 12.58545 5.10 7.900725 1475.766000 0.127920 11.19048 53.85 8.241444 302.522300 0.102336 10.72859 11.04 9.723080 945.921800 0.089544 9.09681 34.52 11.283030 414.659200 0.102336 7.84238 15.13 11.492390 300.886600 0.076752 7.69998 10.98 12.610210 2740.558000 0.089544 7.01982 100.00 12.910370 765.158500 0.063960 6.85728 27.92 13.561180 243.791900 0.153504 6.52963 8.90 14.149930 371.812900 0.102336 6.25924 13.57 15.182550 1607.490000 0.102336 5.83577 58.66 15.806230 690.955800 0.179088 5.60688 25.21 16.673670 482.323700 0.179088 5.31709 17.60 17.068480 365.833300 0.127920 5.19498 13.35 18.200570 206.779900 0.153504 4.87432 7.55 18.933070 395.979900 0.153504 4.68736 14.45 19.293830 277.037400 0.102336 4.60052 10.11 19.924160 251.428800 0.204672 4.45638 9.17 20.583290 158.800000 0.204672 4.31514 5.79 21.951230 220.614300 0.153504 4.04923 8.05 23.477450 72.922780 0.409344 3.78934 2.66 24.511240 99.987140 0.255840 3.63181 3.65 24.954920 117.325600 0.153504 3.56824 4.28 25.993010 108.058000 0.204672 3.42804 3.94 28.257860 72.489400 0.409344 3.15822 2.65 31.063590 95.037750 0.179088 2.87907 3.47 31.653730 62.090590 0.307008 2.82673 2.27 For example, the SCY-078 hippurate Type A has an XRPD pattern comprising one or more peaks at d-spacings of 11.20, 7.02, and 5.84 A. In another example, the SCY-078 hippurate Type A has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.90, 12.6, and 15.18.

In one embodiment, the SCY-078 hippurate Type B has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE B FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.887601 118.925500 0.102336 15.01151 9.56 6.889384 462.675400 0.063960 12.83077 37.19 7.277837 251.176000 0.051168 12.14678 20.19 8.767134 927.066800 0.076752 10.08643 74.52 9.945603 1143.995000 0.102336 8.89377 91.96 10.843500 681.448200 0.102336 8.15925 54.78 11.822440 140.502200 0.127920 7.48575 11.29 12.417990 1244.014000 0.089544 7.12805 100.00 13.714490 435.466500 0.089544 6.45698 35.00 14.608760 1242.496000 0.102336 6.06367 99.88 15.050420 474.015800 0.102336 5.88670 38.10 16.071560 476.890000 0.127920 5.51491 38.33 16.476910 708.831400 0.102336 5.38014 56.98 16.857150 185.689200 0.102336 5.25963 14.93 17.289970 422.781900 0.127920 5.12893 33.99 17.612420 996.474200 0.089544 5.03575 80.10 18.405510 186.288500 0.153504 4.82051 14.97 19.118560 303.851800 0.127920 4.64230 24.43 19.623870 158.474700 0.153504 4.52389 12.74 20.218430 314.377200 0.153504 4.39218 25.27 21.746130 200.050600 0.153504 4.08695 16.08 23.075880 129.668200 0.204672 3.85436 10.42 23.853540 106.856400 0.204672 3.73044 8.59 25.372290 96.670350 0.204672 3.51048 7.77 29.216870 66.396300 0.230256 3.05670 5.34 32.714200 31.053470 0.614016 2.73748 2.50 For example, the SCY-078 hippurate Type B has an XRPD pattern comprising one or more peaks at d-spacings of 8.90, 7.13, and 6.10 A. In another example, the SCY-078 hippurate Type B has an XRPD pattern comprising one or more peaks at degrees 2 theta of 9.95, 12.42, and 14.61.

In one embodiment, the SCY-078 hippurate Type C has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE C Pos. FWHM [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 3.027072 11818.150000 0.051168 29.18766 100.00 5.916137 351.270000 0.102336 14.93916 2.97 6.916698 947.910600 0.102336 12.78016 8.02 7.251646 624.847700 0.076752 12.19059 5.29 8.761702 1828.733000 0.076752 10.09268 15.47 9.962105 2404.236000 0.102336 8.87907 20.34 10.897800 1593.408000 0.102336 8.11872 13.48 11.868550 552.254500 0.102336 7.45677 4.67 12.432300 2880.441000 0.127920 7.11988 24.37 12.857840 525.634600 0.076752 6.88518 4.45 13.091360 511.764400 0.115128 6.76288 4.33 13.709840 1112.219000 0.102336 6.45916 9.41 14.555290 3086.294000 0.153504 6.08582 26.11 14.984610 1215.693000 0.102336 5.91241 10.29 15.341160 506.870800 0.153504 5.77579 4.29 16.136210 1315.742000 0.089544 5.49296 11.13 16.453540 1710.358000 0.153504 5.38772 14.47 16.897030 606.324900 0.102336 5.24730 5.13 17.280760 1171.798000 0.127920 5.13164 9.92 17.591700 2258.867000 0.102336 5.04163 19.11 18.190770 538.754800 0.127920 4.87692 4.56 18.425670 516.831300 0.179088 4.81528 4.37 19.151570 950.084500 0.102336 4.63437 8.04 19.602330 487.956400 0.127920 4.52881 4.13 20.234760 861.917600 0.153504 4.38867 7.29 20.860030 424.598600 0.153504 4.25851 3.59 21.725360 459.496200 0.307008 4.09081 3.89 22.532320 498.240700 0.102336 3.94610 4.22 23.078810 380.947900 0.127920 3.85388 3.22 23.551950 208.488500 0.409344 3.77752 1.76 23.874020 377.598600 0.102336 3.72728 3.20 25.381750 351.553600 0.102336 3.50919 2.97 25.844490 207.070300 0.204672 3.44740 1.75 27.188450 192.463400 0.153504 3.27997 1.63 27.681830 144.369000 0.307008 3.22262 1.22 29.319670 172.870900 0.511680 3.04622 1.46 30.833510 86.432220 0.307008 2.90002 0.73 34.979000 90.330020 0.204672 2.56525 0.76 35.588330 69.479680 0.307008 2.52271 0.59 37.270360 55.666410 0.307008 2.41264 0.47 For example, the SCY-078 hippurate Type C has an XRPD pattern comprising one or more peaks at d-spacings of 29.19, 8.88, 7.12, and 6.09 Å. In another example, the SCY-078 hippurate Type C has an XRPD pattern comprising one or more peaks at degrees 2 theta of 3.03, 9.96, 12.43, and 14.56.

The present disclosure further relates to fumarate salts of SCY-078, such as SCY-078 fumarate Type A and SCY-078 fumarate Type B. In one embodiment, the SCY-078 fumarate Type A has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE D FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 7.747007 167.350400 0.153504 11.41218 2.00 8.493147 8373.572000 0.102336 10.41119 100.00 9.346397 181.715800 0.204672 9.46257 2.17 9.931064 167.355100 0.153504 8.90676 2.00 10.442940 371.960600 0.089544 8.47130 4.44 10.706620 463.482500 0.076752 8.26326 5.54 11.231160 490.618200 0.153504 7.87848 5.86 13.030860 311.426800 0.102336 6.79415 3.72 13.700070 569.254300 0.102336 6.46374 6.80 14.895760 234.024600 0.153504 5.94748 2.79 15.204970 269.752200 0.153504 5.82722 3.22 16.350790 434.953000 0.127920 5.42135 5.19 16.976580 3015.489000 0.115128 5.22289 36.01 17.726110 1152.135000 0.230256 5.00370 13.76 18.205910 303.920500 0.102336 4.87290 3.63 18.863510 267.939100 0.153504 4.70449 3.20 20.164360 63.804870 0.409344 4.40383 0.76 20.898390 184.877000 0.102336 4.25078 2.21 21.419940 168.417300 0.102336 4.14844 2.01 22.228150 318.867400 0.127920 3.99940 3.81 23.936960 77.330220 0.307008 3.71763 0.92 25.533030 318.681700 0.089544 3.48874 3.81 26.114530 59.303240 0.204672 3.41236 0.71 26.883130 111.136200 0.204672 3.31652 1.33 30.876670 38.684340 0.614016 2.89607 0.46 For example, the SCY-078 fumarate Type A has an XRPD pattern comprising one or more peaks at d-spacings of 10.41, 5.22, and 5.00 A. In another example, the SCY-078 fumarate Type A has an XRPD pattern comprising one or more peaks at degrees 2 theta of 8.49, 16.98, and 17.73.

In one embodiment, the SCY-078 fumarate Type B has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE E FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.449312 94.567020 0.307008 16.21786 19.92 6.318422 110.456500 0.409344 13.98888 23.26 9.799620 153.670200 0.614016 9.02593 32.36 10.577440 403.264100 0.255840 8.36388 84.93 10.995710 322.682700 0.089544 8.04665 67.96 11.970210 133.555200 0.307008 7.39367 28.13 13.136230 472.855300 0.102336 6.73989 99.58 13.551710 408.076200 0.102336 6.53417 85.94 14.201760 320.510900 0.204672 6.23651 67.50 15.712210 472.732700 0.076752 5.64022 99.56 16.216750 474.828900 0.076752 5.46586 100.00 16.849640 211.687300 0.204672 5.26195 44.58 20.391740 103.586500 0.358176 4.35524 21.82 21.343910 97.997770 0.409344 4.16305 20.64 28.564840 34.739620 0.614016 3.12498 7.32 For example, the SCY-078 fumarate Type B has an XRPD pattern comprising one or more peaks at d-spacings of 8.36, 6.74, 6.53, 5.64, and 5.47 Å. In another example, the SCY-078 fumarate Type B has an XRPD pattern comprising one or more peaks at degrees 2 theta of 10.58, 13.14, 13.55, 15.71, and 16.22.

The present disclosure further relates to glycolate salts of SCY-078. In one embodiment, the SCY-078 glycolate has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE F FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 7.879599 291.814000 0.127920 11.22044 54.74 8.983378 233.086200 0.204672 9.84412 43.72 9.855934 117.677600 0.153504 8.97448 22.07 11.483230 269.580500 0.204672 7.70610 50.56 12.311460 163.106800 0.358176 7.18949 30.59 14.259570 285.813400 0.179088 6.21136 53.61 14.651000 437.366100 0.102336 6.04628 82.04 15.433320 533.138100 0.102336 5.74151 100.00 16.892280 103.441500 0.614016 5.24877 19.40 18.826490 177.863500 0.204672 4.71365 33.36 20.401140 101.236100 0.307008 4.35325 18.99 21.743970 54.436950 0.614016 4.08735 10.21 24.981860 29.298130 0.614016 3.56445 5.50 For example, the SCY-078 glycolate has an XRPD pattern comprising one or more peaks at d-spacings of 11.22, 6.21, 6.05, and 5.74 A. In another example, the SCY-078 glycolate has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.88, 14.26, 14.65, and 15.43.

The present disclosure further relates to mesylate salts of SCY-078. In one embodiment, the SCY-078 mesylate has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE G FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.321569 44.016770 0.307008 16.60688 3.75 6.654286 233.467600 0.076752 13.28355 19.89 8.041747 243.835400 0.204672 10.99455 20.77 9.224843 236.712900 0.153504 9.58698 20.16 10.179890 547.128100 0.089544 8.68960 46.60 10.532080 592.551800 0.089544 8.39980 50.47 11.692820 225.932500 0.409344 7.56843 19.24 12.670270 361.926000 0.102336 6.98668 30.83 14.316750 537.652200 0.102336 6.18668 45.80 14.751260 1174.011000 0.102336 6.00541 100.00 15.645660 347.928600 0.204672 5.66406 29.64 16.537910 485.586600 0.179088 5.36043 41.36 17.477180 328.731900 0.127920 5.07441 28.00 18.838670 252.134300 0.307008 4.71063 21.48 19.613670 351.448500 0.153504 4.52622 29.94 21.008230 254.102200 0.204672 4.22880 21.64 22.068870 130.646600 0.307008 4.02791 11.13 23.475460 151.601600 0.204672 3.78965 12.91 25.592960 130.952000 0.153504 3.48071 11.15 For example, the SCY-078 mesylate has an XRPD pattern comprising one or more peaks at d-spacings of 10.99, 6.99, and 6.01 Å. In another example, the SCY-078 mesylate has an XRPD pattern comprising one or more peaks at degrees 2 theta of 8.04, 12.67, and 14.75.

The present disclosure further relates to calcium salts of SCY-078. In one embodiment, the SCY-078 Calcium has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE H FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.330948 1222.647000 0.063960 16.57768 100.00 8.684942 82.143680 0.307008 10.18170 6.72 9.624508 215.229600 0.127920 9.18975 17.60 10.625810 286.722000 0.153504 8.32591 23.45 13.358050 110.336200 0.307008 6.62846 9.02 14.092620 101.325400 0.307008 6.28456 8.29 15.952080 1188.492000 0.153504 5.55594 97.21 16.282720 334.685300 0.153504 5.44386 27.37 17.853110 104.842700 0.153504 4.96839 8.58 19.638160 74.407610 0.307008 4.52063 6.09 29.434800 171.668200 0.204672 3.03457 14.04 30.178030 59.353870 0.153504 2.96150 4.85 31.428330 37.765730 0.307008 2.84648 3.09 For example, the SCY-078 calcium has an XRPD pattern comprising one or more peaks at d-spacings of 16.58, 5.56, and 5.44 A. In another example, the SCY-078 calcium has an XRPD pattern comprising one or more peaks at degrees 2 theta of 5.33, 15.95, and 16.28.

The present disclosure further relates to citrate salts of SCY-078, such as SCY-078 citrate Type A, Type B, Type E, Type F, Type M, Type N, Type 0, Type Q, Type R, and Type S. In one embodiment, the citrate salt of SCY-078 comprises at least one of Type A, Type B, Type E, Type F, Type M, Type N, Type 0, Type Q, Type R, and Type S.

The present disclosure further relates to a citrate salt of SCY-078 comprising Type A. In one embodiment, the citrate salt of SCY-078 consists essentially of Type A. In another embodiment, the citrate salt of SCY-078 comprises at least 98% Type A. In a further embodiment, the citrate salt of SCY-078 comprises at least 99% Type A.

In one embodiment, the SCY-078 citrate Type A is stable for at least 1 week when stored at 60° C. In another embodiment, the SCY-078 citrate Type A is stable for at least 1 week when stored at 25° C. and 60% relative humidity. In a further embodiment, the SCY-078 citrate Type A is stable for at least 1 week when stored at 40° C. and 75% relative humidity.

In a further embodiment, the SCY-078 citrate Type A has an equilibrium solubility of 38 mg/mL in non-buffered water at ambient temperature. In yet another embodiment, the SCY-078 citrate Type A has an approximate solubility of from 40 mg/mL to 42 mg/mL at room temperature in at least one solvent selected from methanol, isopropyl alcohol, acetic acid, tetrahydrofuran, 2 methyl-tetrahydrofuran, 1,4-dioxane, n-methyl-2-pyrrolidone, dimethyl sulfoxide, and dimethylacetamide. In still another embodiment, the SCY-078 citrate Type A has a water sorption of 6% at 25° C. and 80% relative humidity as determined by DVS.

In one embodiment, the SCY-078 citrate Type A has a kinetic solubility of 4 mg/mL at 4 hours in dextrose buffer at pH 5.5. In another embodiment, the SCY-078 citrate Type A has a kinetic solubility of 8 mg/mL at 24 hours in dextrose buffer at pH 5.5. In a further embodiment, the SCY-078 citrate Type A has a kinetic solubility of 5 mg/mL at 4 hours in phosphate buffer at pH 6.0. In still another embodiment, the SCY-078 citrate Type A has a kinetic solubility of 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.

In one embodiment, the SCY-078 citrate Type A has a kinetic solubility of 21 mg/mL at 1 hour in SGF media. In another embodiment, the SCY-078 citrate Type A has a kinetic solubility of 4 mg/mL at 24 hours in FeSSIF media. In yet another embodiment, the SCY-078 citrate Type A has a kinetic solubility of 10 mg/mL at 1 hour in FaSSIF media. In a further embodiment, the SCY-078 citrate Type A has a kinetic solubility of 21 mg/mL at 4 hours in FaSSIF media.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type A. In one embodiment, the SCY-078 citrate Type A has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE I Pos. FWHM [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.400273 434.322700 0.102336 16.36502 3.34 7.453872 13000.820000 0.191880 11.86031 100.00 9.201639 691.948300 0.204672 9.61110 5.32 10.831710 404.555000 0.153504 8.16811 3.11 11.485080 936.115200 0.179088 7.70486 7.20 12.491050 954.805500 0.179088 7.08652 7.34 13.191360 1776.320000 0.204672 6.71184 13.66 15.020350 1342.537000 0.204672 5.89842 10.33 15.664830 532.278900 0.179088 5.65717 4.09 15.955570 613.057500 0.127920 5.55474 4.72 16.751250 951.729000 0.153504 5.29264 7.32 17.978130 170.323300 0.204672 4.93412 1.31 19.591770 472.971000 0.204672 4.53123 3.64 22.213400 146.982900 0.204672 4.00202 1.13 23.845740 34.469910 0.614016 3.73164 0.27 25.160050 117.741100 0.307008 3.53961 0.91 28.761350 129.234400 0.255840 3.10407 0.99 30.356250 332.945100 0.230256 2.94452 2.56 32.317870 87.151140 0.307008 2.77014 0.67 34.725480 74.664570 0.511680 2.58339 0.57 For example, the SCY-078 citrate Type A has an XRPD pattern comprising one or more peaks at d-spacings of 11.86, 7.70, 7.09, 6.71, 5.90, and 5.29 Å. In another example, the SCY-078 citrate Type A has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.45, 11.49, 12.49, 13.19, 15.02, and 16.75.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type B. In one embodiment, the SCY-078 citrate Type B has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE J FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.561437 214.772000 0.127920 15.89114 18.87 6.920576 1138.274000 0.115128 12.77301 100.00 9.319721 77.009080 0.307008 9.48959 6.77 11.144180 155.075600 0.153504 7.93978 13.62 11.729970 201.281900 0.153504 7.54455 17.68 13.405240 187.402700 0.358176 6.60523 16.46 15.225970 237.746900 0.204672 5.81923 20.89 16.813690 449.144100 0.153504 5.27312 39.46 18.219030 148.764600 0.204672 4.86942 13.07 19.324790 108.017600 0.153504 4.59322 9.49 20.531330 143.254500 0.127920 4.32594 12.59 23.721410 34.728650 0.307008 3.75092 3.05 26.000800 68.151450 0.204672 3.42703 5.99 29.343000 18.852780 0.614016 3.04385 1.66 For example, the SCY-078 citrate Type B has an XRPD pattern comprising one or more peaks at d-spacings of 15.89, 12.77, 7.54, 5.82, and 5.27 Å. In another example, the SCY-078 citrate Type B has an XRPD pattern comprising one or more peaks at degrees 2 theta of 5.56, 6.92, 11.73, 15.23, and 16.81.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type E. In one embodiment, the SCY-078 citrate Type E has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE K FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.524293 92.779970 0.307008 15.99790 5.74 7.256628 1616.341000 0.179088 12.18224 100.00 11.438900 216.111400 0.281424 7.73586 13.37 14.135060 246.012400 0.255840 6.26579 15.22 15.755470 336.295500 0.255840 5.62483 20.81 16.331430 208.920100 0.255840 5.42773 12.93 17.088060 99.712520 0.409344 5.18907 6.17 21.127980 46.130650 0.614016 4.20511 2.85 31.562360 23.421260 0.614016 2.83470 1.45 For example, the SCY-078 citrate Type E has an XRPD pattern comprising one or more peaks at d-spacings of 12.18, 7.74, 6.27, 5.62, and 5.43 A. In another example, the SCY-078 citrate Type E has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.26, 11.44, 14.14, 15.76, and 16.33.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type F. In one embodiment, the SCY-078 citrate Type F has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE L FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 3.633823 273.473300 0.204672 24.31535 100.00 8.094996 83.291080 0.307008 10.92235 30.46 14.004250 57.266020 0.818688 6.32402 20.94 17.742840 88.241520 0.307008 4.99902 32.27 For example, the SCY-078 citrate Type F has an XRPD pattern comprising one or more peaks at d-spacings of 24.32 and 5.00 Å. In another example, the SCY-078 citrate Type F has an XRPD pattern comprising one or more peaks at degrees 2 theta of 3.63 and 17.74.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type M. In one embodiment, the SCY-078 citrate Type M has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE M FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.572099 251.586000 0.179088 15.86076 9.89 7.341430 2543.546000 0.332592 12.04171 100.00 9.506812 193.442600 0.307008 9.30326 7.61 11.507120 612.058600 0.281424 7.69016 24.06 12.151790 217.504900 0.255840 7.28359 8.55 14.166310 475.291100 0.179088 6.25204 18.69 15.796090 798.112100 0.255840 5.61046 31.38 16.373100 704.236700 0.179088 5.41401 27.69 17.342680 236.560600 0.511680 5.11346 9.30 18.264100 127.099200 0.307008 4.85751 5.00 20.028560 111.330700 0.307008 4.43338 4.38 21.230190 166.125100 0.255840 4.18509 6.53 22.124240 151.032300 0.358176 4.01795 5.94 23.019390 107.550400 0.307008 3.86369 4.23 25.286220 144.601600 0.511680 3.52223 5.69 27.656070 79.447100 0.358176 3.22556 3.12 28.430390 56.622940 0.409344 3.13945 2.23 29.646340 75.432070 0.614016 3.01339 2.97 32.376530 74.417430 0.307008 2.76525 2.93 36.534050 34.760060 0.614016 2.45955 1.37 38.139080 26.017290 0.614016 2.35966 1.02 For example, the SCY-078 citrate Type M has an XRPD pattern comprising one or more peaks at d-spacings of 12.04, 7.69, 6.25, 5.61, and 5.41 A. In another example, the SCY-078 citrate Type M has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.34, 11.51, 14.17, 15.80, and 16.37.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type N. In one embodiment, the SCY-078 citrate Type N has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE N FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 5.410849 486.098000 0.076752 16.33306 9.13 7.067553 5326.844000 0.089544 12.50771 100.00 10.838060 670.175200 0.063960 8.16333 12.58 11.383920 1260.568000 0.089544 7.77310 23.66 11.924900 315.766000 0.153504 7.42166 5.93 12.367310 352.822800 0.153504 7.15714 6.62 12.923310 1574.956000 0.089544 6.85044 29.57 14.132120 1282.157000 0.102336 6.26708 24.07 15.162450 1604.350000 0.102336 5.84346 30.12 16.256930 1496.153000 0.089544 5.45244 28.09 16.676790 891.116500 0.115128 5.31610 16.73 16.898590 608.961300 0.102336 5.24682 11.43 17.769210 633.106100 0.127920 4.99166 11.89 18.512560 1119.245000 0.102336 4.79287 21.01 20.764100 264.835400 0.102336 4.27797 4.97 21.599360 277.781400 0.127920 4.11439 5.21 22.726850 204.007000 0.102336 3.91276 3.83 23.066060 336.013100 0.153504 3.85598 6.31 24.489610 243.365100 0.127920 3.63497 4.57 28.491330 175.736200 0.179088 3.13287 3.30 30.668850 84.372280 0.307008 2.91522 1.58 33.097360 34.363080 0.614016 2.70666 0.65 36.308500 40.510880 0.716352 2.47431 0.76 For example, the SCY-078 citrate Type N has an XRPD pattern comprising one or more peaks at d-spacings of 12.51, 7.77, 6.85, 6.27, 5.84, 5.45, and 4.79 Å. In another example, the SCY-078 citrate Type N has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.07, 11.38, 12.92, 14.13, 15.16, 16.26, and 18.51.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type O In one embodiment, the SCY-078 citrate Type O has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE O FWHM Pos. [°2Th.] Height [cts] Left [°2Th.] d-spacing [Å] Rel. Int. [%] 3.214240 359.101800 0.409344 27.48844 9.56 5.562890 564.632100 0.102336 15.88699 15.03 7.082335 3757.717000 0.115128 12.48164 100.00 11.908250 1208.103000 0.089544 7.43200 32.15 14.197590 602.552700 0.115128 6.23833 16.04 16.178670 447.137400 0.179088 5.47864 11.90 16.755170 956.290800 0.115128 5.29141 25.45 28.567280 48.759020 0.307008 3.12472 1.30 For example, the SCY-078 citrate Type 0 has an XRPD pattern comprising one or more peaks at d-spacings of 12.48, 7.43, and 5.29 A. In another example, the SCY-078 citrate Type O has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.08, 11.91, and 16.76.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type Q. In one embodiment, the SCY-078 citrate Type Q has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE P FWHM Left Pos. [°2Th.] Height [cts] [°2Th.] d-spacing [Å] Rel. Int. [%] 5.686347 449.970600 0.102336 15.54234 13.51 6.300879 3329.599000 0.140712 14.02779 100.00 6.890776 1871.585000 0.076752 12.82818 56.21 8.441730 95.233120 0.153504 10.47449 2.86 9.785571 136.396600 0.153504 9.03885 4.10 11.334590 1386.986000 0.140712 7.80682 41.66 11.733060 826.632000 0.102336 7.54257 24.83 12.939760 265.781600 0.409344 6.84177 7.98 13.691820 190.778000 0.153504 6.46762 5.73 14.156830 332.781500 0.153504 6.25620 9.99 14.496570 455.453300 0.102336 6.11034 13.68 15.135910 594.105600 0.153504 5.85365 17.84 15.903400 540.737100 0.127920 5.57284 16.24 17.010910 1588.263000 0.127920 5.21243 47.70 17.296950 476.914900 0.127920 5.12687 14.32 18.962100 570.585000 0.204672 4.68025 17.14 20.190720 395.466100 0.102336 4.39814 11.88 20.646480 601.591200 0.153504 4.30207 18.07 21.298380 208.197100 0.153504 4.17185 6.25 22.025220 160.183700 0.307008 4.03579 4.81 22.719750 205.611500 0.204672 3.91397 6.18 23.633070 128.288000 0.307008 3.76474 3.85 25.991160 157.744000 0.204672 3.42828 4.74 27.462080 37.389280 0.307008 3.24790 1.12 28.950740 597.140100 0.140712 3.08419 17.93 34.085010 29.835660 0.511680 2.63046 0.90 For example, the SCY-078 citrate Type Q has an XRPD pattern comprising one or more peaks at d-spacings of 14.03, 12.83, 7.81, 7.54, and 5.21 Å. In another example, the SCY-078 citrate Type Q has an XRPD pattern comprising one or more peaks at degrees 2 theta of 6.30, 6.89, 11.33, 11.73, and 17.01.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type R. In one embodiment, the SCY-078 citrate Type R has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE Q FWHM Left Pos. [°2Th.] Height [cts] [°2Th.] d-spacing [Å] Rel. Int. [%] 6.143884 611.904200 0.153504 14.38589 100.00 11.248800 143.606900 0.255840 7.86616 23.47 14.059410 351.488100 0.204672 6.29933 57.44 14.636960 372.993000 0.204672 6.05205 60.96 16.413500 550.672100 0.102336 5.40078 89.99 17.742000 362.377400 0.614016 4.99926 59.22 19.697290 248.048100 0.307008 4.50719 40.54 22.159300 133.589200 0.409344 4.01167 21.83 30.197650 27.706020 0.614016 2.95963 4.53 For example, the SCY-078 citrate Type R has an XRPD pattern comprising one or more peaks at d-spacings of 14.39, 6.05, 5.40, and 5.00 Å. In another example, the SCY-078 citrate Type R has an XRPD pattern comprising one or more peaks at degrees 2 theta of 6.14, 14.64, 16.41, and 17.74.

The present disclosure further relates to a citrate salt of SCY-078 comprising SCY-078 citrate Type S. In one embodiment, the SCY-078 citrate Type S has an XRPD pattern comprising peaks at one or more of the following locations:

TABLE R FWHM Left Pos. [°2Th.] Height [cts] [°2Th.] d-spacing [Å] Rel. Int. [%] 5.512446 1020.013000 0.089544 16.03226 16.16 7.296105 6310.710000 0.153504 12.11641 100.00 8.443163 252.219900 0.230256 10.47272 4.00 11.057440 582.228600 0.179088 8.00186 9.23 12.004950 2714.326000 0.166296 7.37235 43.01 14.346070 536.999100 0.204672 6.17410 8.51 16.812800 1626.861000 0.127920 5.27340 25.78 19.482230 106.142000 0.358176 4.55646 1.68 22.188280 88.048920 0.409344 4.00650 1.40 24.046320 53.183810 0.818688 3.70096 0.84 For example, the SCY-078 citrate Type S has an XRPD pattern comprising one or more peaks at d-spacings of 16.03, 12.12, 7.37, and 5.27 A. In another example, the SCY-078 citrate Type S has an XRPD pattern comprising one or more peaks at degrees 2 theta of 5.51, 7.30, 12.00, and 16.81.

The present disclosure further relates to a method for preparing a pharmaceutically acceptable salt of SCY-078 comprising combining at least components: (i) a free base of SCY-078; (ii) a weak organic acid; and (iii)a liquid carrier. The weak organic acid may be chosen from those known in the art. In one embodiment, the weak organic acid is selected from citric acid, fumaric acid, methanesulfonic acid, and hippuric acid. In another embodiment, the weak organic acid is citric acid. In a further embodiment, the liquid carrier is a solvent or solvent mixture, and at least one of the free base of SCY-078 and the weak organic acid is soluble in the solvent or solvent mixture. In still another embodiment, the liquid carrier comprises at least one of ethanol, isopropyl alcohol, acetonitrile, acetone, ethyl acetate, and tetrahydrofuran/water mixture. In yet another embodiment, the liquid carrier comprises ethanol. In one embodiment, the method further comprises combining (iv) an anti-solvent. In another embodiment, the anti-solvent comprises N-heptane.

In one embodiment, the method further comprises agitating the combination of at least components (i)-(iii). In a further embodiment, the method further comprises agitating the combination of at least components (i)-(iii) for at least 24 hours. In another embodiment, the method further comprises agitating and heating the combination of at least components (i)-(iii). In a further embodiment, the method further comprises agitating and heating the combination of at least components (i)-(iii) to a temperature of from 40° C. to 60° C. In still another embodiment, the method further comprises agitating and heating the combination of at least components (i)-(iii) to a temperature of from 40° C. to 60° C. for at least 60 minutes.

In one embodiment, the method further comprises agitating and heating the combination of at least components (i)-(iii) and adding to the combination of at least components (i)-(iii) an anti-solvent after at least 14 hours. In yet another embodiment, the anti-solvent is N-heptane.

In one embodiment, the method further comprises agitating and heating the combination of at least components (i)-(iii); adding to the combination of at least components (i)-(iii) an anti-solvent after at least 14 hours; and cooling the combination of at least components (i)-(iii). In a further embodiment, the cooling is from 0° C. to 20° C. In another embodiment, the cooling is from 0° C. to 20° C. at a rate of 0.25° C./min.

The present disclosure further relates to methods for preparing citrate Type A salt of SCY-078. In one embodiment, the method comprises desolvating at least one of Type B, Type N, and Type Q citrate salt of SCY-078. In a further embodiment, the desolvating comprises drying under nitrogen. In yet another embodiment, the desolvating comprises drying under vacuum filtration.

The present disclosure additionally relates to pharmaceutical compositions comprising a pharmaceutically acceptable salt of SCY-078, and a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier may be chosen from, among other things, one or more of the following: water, saline solutions, buffers, and alcohols. In one embodiment, the pharmaceutically acceptable salt in the pharmaceutical composition is selected from citrate, hippurate, mesylate, and fumarate. In yet another embodiment, the pharmaceutically acceptable salt is a citrate salt. In still another embodiment, the pharmaceutically acceptable salt of SCY-078 is SCY-078 citrate Type A.

According to certain embodiments, the pharmaceutically acceptable salt of SCY-078 may consist essentially of a specified crystal form. According to certain embodiments, the pharmaceutically acceptable salt of SCY-078 may comprise a specified crystal in combination with one or more other crystal forms. The pharmaceutically acceptable salt of SCY-078 may, for example, contain a specified crystal form together with less than 10% of another crystal form(s), such as less than 5%, less than 2%, or less than 1%.

In one embodiment, the pharmaceutical composition is made by dissolving the pharmaceutically acceptable salt of SCY-078 in a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier may be chosen from, among other things, one or more of the following: water, saline solutions, buffers, and alcohols. In another embodiment, the pharmaceutical composition is suitable for injection into a human. In a further embodiment, the pharmaceutical composition is suitable for intravenous injection into a human. In another embodiment, the pharmaceutically acceptable salt is a citrate salt. In still another embodiment, the pharmaceutically acceptable salt of SCY-078 is SCY-078 citrate Type A.

The present disclosure further relates to methods of preparing a pharmaceutical composition comprising pharmaceutically acceptable salts of SCY-078, and a pharmaceutically acceptable carrier. The pharmaceutically acceptable carrier may be chosen from, among other things, one or more of the following: water, saline solutions, buffers, and alcohols. In one embodiment, the pharmaceutically acceptable salt of SCY-078 is dissolved in the pharmaceutically acceptable carrier within 1 hour. In another embodiment, the pharmaceutically acceptable salt of SCY-078 is dissolved in the pharmaceutically acceptable carrier within 24 hours. In a further embodiment, the pharmaceutically acceptable salt is a citrate salt. In still another embodiment, the pharmaceutically acceptable salt of SCY-078 is SCY-078 citrate Type A.

The present disclosure additionally relates to methods of treating a fungal infection in a patient in need thereof. For example, the methods include treating Invasive Candidiasis and Invasive Aspergillosis. In one embodiment, the method comprises administering to the patient in need thereof a pharmaceutical composition comprising an effective amount of a pharmaceutically acceptable salt of SCY-078. In another embodiment, the pharmaceutically acceptable salt is a citrate salt. In a further embodiment, the pharmaceutically acceptable salt of SCY-078 is SCY-078 citrate Type A. In another embodiment, the pharmaceutical composition is suitable for injection into a human. In yet another embodiment, the pharmaceutical composition is suitable for intravenous injection into a human.

EXAMPLES

Methods and Materials

The following describes the materials and methods used for all examples unless otherwise stated.

TABLE 1 Abbreviation/ Type Acronym Full Name/Description Solvent ACN Acetonitrile DCM Dichloromethane DMA Dimethylamine DMAc Dimethylacetamide DMSO Dimethyl sulfoxide EtOH Ethanol EtOAc Ethyl Acetate IPA Isopropyl alcohol IPAc Isopropyl acetate MeOH Methanol MIBK Methyl isobutyl ketone MTBE Methyl tert-butyl ether NMP N-methyl-2-pyrrolidone THF Tetrahydrofuran Bio-Relevant SGF Simulated gastric fluid Media FaSSIF Fasted state simulated intestinal fluid FeSSIF Fed state simulated intestinal fluid Techniques DSC Differential scanning calorimetry DVS Dynamic Vapor Sorption HPLC High Performance Liquid Chromatography TGA Thermogravimetric analysis XRPD X-ray power diffraction Other FB Freebase NF New form RH Relative humidity RT Room temperature

X-Ray Powder Diffraction (XRPD)—To perform XRPD analysis, a PANanalytical Empryean X-ray powder diffractometer was used. The typical XRPD parameters used are listed below. Data Viewer (version 1.4a) from PANanalytic was used for analysis.

X-Ray Wavelength Cu, kα, Kα1 (Å): 1.540598, Kα2 (Å): 1.544426 Kα2/Kα1 intensity ratio: 0.50 X-Ray tube setting 45 kV, 40 mA Divergence split Automatic Scan mood Continuous Scan range (°2 TH) 3°-40° Step size (°2 TH) 0.0130 or 0.0170 Scan speed (°/min) ~10

Differential Scanning Calorimetry (DSC)—DSC was performed with a TA Q2000 DSC from TA Instruments. To perform DSC, the sample was ramped from room temperature to the desired temperature at a heating rate of 10° C./min, using N₂ as the purge gas and with the pan crimped. Universal Analysis 2000 (TA Instruments) was used to analyze the results.

Thermogravimetric Analysis (TGA)—TGA was performed with a TA Q500/Q5000 TGA from TA Instruments. To perform TGA, the sample was ramped from room temperature to the desired temperature at a heating rate of 10° C./min, using N₂ as the purge gas. Universal Analysis 2000 (TA Instruments) was used to analyze the results. The temperature was calibrated using nickel and the weight using TA-supplied standard weights and verified against calcium oxalate monohydrate dehydration and decomposition.

Dynamic Vapor Sorption (DVS)—The term “DVS” means the procedure described in below. The relative humidity at 25° C. was calibrated against deliquescence point of LiCl, Mg(NO₃)₂ and KCl.

Temperature 25° C. Gas Flow and Rate N₂, 200 mL/min dm/dt 0.002%/min Min. dm/dt stability duration 10 min Max. equilibrium time 180 min RH range 95% RH-0% RH-95% RH RH step size 10% (90% RH-0% RH-90% RH) 5% (90% RH-95% RH-90% RH)

The DVS of SCY-078 salts were tested according to the above method, using a 10-20 mg sample size. The DVS was measured using a Surface Measurement Systems (SMS) DVS Intrinsic.

High Power Liquid Chromatography (HPLC) Method—An Agilent 1260 HPLC with DAD detector was utilized to test solubility or to test purity and stability. For all compounds tested other than the trifluoroacetate salts (Type A and B) and the HCl Type I and II salts, the conditions and parameters used for measuring solubility are shown in Table 2A and for measuring stability are showing in Table 3A. The conditions and parameters used for the solubility of the trifluoroacetate salts (Type A and B) and the HCl Type I and II salts are shown in Table 2B and for stability are shown in Table 3B. System suitability was tested by injecting standard solutions five times in each sample sequence, and the relative standard deviation of the peak areas was less than 2%.

TABLE 2A Column 53#: Eclipse plus C18, 4.6 * 150 mm, 3.5 μm Mobile Phase A: 0.1% HClO₄ in H₂OB: ACN Time (mins) % A % B Gradient Table 0 70 30 1 70 30 7 5 95 8 5 95 8.1 70 30 12 70 30 Flow Rate 1.0 mL/min Injection Volume 10 μL Detector Wavelength 255 nm Run Time 12 min Column Temperature 40° C. Autosampler RT Temperature

TABLE 2B Column Waters C18 110A, 4.6 * 150 mm, 5 μm Mobile Phase A: 0.1% TFA in H₂O; B: 0.1% TFA in acetonitrile Time (mins) % B Gradient Table 0 5 1 5 9 95 11 95 11.1 5 15 5 Flow Rate 1.0 mL/min Injection Volume 10 μL Detector Wavelength UV at 255 nm Run Time 15 min Column Temperature 40° C. Autosampler Temperature RT

TABLE 3A Column Phenomenex, Gemini C18, 4.6 × 150 mm, 3 mm Mobile Phase A: 0.1% HClO₄ in H₂O B: ACN Time (mins) % A % B Gradient Table 0.0 63 37 5.0 43 57 15.0 38 62 20.0 10 90 30.0 10 90 31.0 63 37 36.0 63 37 Flow Rate 1.0 mL/min Injection Volume 10 μL Detector Wavelength UV at 210 min Run Time 36.0 min Column Temperature 40° C. Sampler Temperature RT Diluent ACN

TABLE 3B Column Waters C18 110A, 4.6 * 150 mm, 5 μm Mobile Phase A: 0.1% TFA in H₂O; B: 0.1% TFA in acetonitrile Time (mins) % B Gradient Table 0.0 5 23 95 27 95 27.1 5 30 5 Flow Rate 1.0 mL/min Injection Volume 10 μL Detector Wavelength 270 min Run Time 30.0 min Column Temperature 40° C. Sampler Temperature RT

SGF Media Preparation—The term “SGF media” means a solution prepared according to the following method. Sodium chloride (0.2 g) and Triton X-100 (0.1 g) were combined in a 100 mL flask. Then deionized water was added. The mixture was stirred until all solids were dissolved. Then 12 N HCl (200 μL) was added and the pH value was checked with a pH meter. The pH was adjusted to 1.8 with 1N HCl or 1N NaOH. Once the desired pH was established, the solution was diluted to volume with deionized water.

FaSSIF Media Preparation—The term “FaSSIF media” means a solution prepared according to the following method. A FaSSIF Dissolving Buffer was prepared by dissolving maleic acid (0.222 g) in 45 mL of purified water. The pH was adjusted to exactly 6.4 using 1N NaOH.

FaSSIF media was prepared by adding sodium taurocholate (0.161 g), sodium chloride (0.398 g), and lecithin (0.0156 g) into a 100-mL volumetric flask. Then 40 mL of deionized water was added. The solution was sonicated until clear. Next 45 mL of the FaSSIF Dissolving Buffer was added. The pH was adjusted to 6.5 with 1N NaOH or 1N HCl. Once the desired pH was reached, the solution was diluted to volume with deionized water.

An alternative media (“FaSSIF alternative media”) was used to study the trifluoroacetate salts (Type A and B) and the HCl Type I salt. More specifically, the media was prepared by weighing 0.17 g of sodium phosphate monobasic (NaH2PO4, anhydrous), 0.021 g of sodium hydroxide, and 0.31 g of sodium chloride into a 50-mL volumetric flask and was dissolve with approximately 48 mL of purified water. The pH was adjusted to exactly 6.5 using 1 M HCl or 1 M NaOH and diluted to volume with purified water. 0.11 g of SIF powder was then added, stirred and sonicated until all the powder was completely dissolved. The solution was equilibrated for 2 hours at RT before use. The solution can be stored at RT for 48 hours or 4° C. for 7 days and should be equilibrated to RT before use

FeSSIF Preparation—The term “FeSSIF media” means a solution prepared according to the following method. A FeSSIF Dissolving Buffer was prepared by dissolving maleic acid (0.638 g) and NaCl (0.728 g) in 100 mL of purified water. The pH was adjusted to exactly 5.8 using 1N NaOH or 1N HCl.

FeSSIF media was prepared by adding sodium taurocholate (0.269 g), lecithin (0.078 g), sodium oleate (0.012 g), and glyceryl monooleate (0.089) into a 50-mL flask. Then 2.5 mL of the FeSSIF Dissolving Buffer was added. The solution was sonicated. An additional 12.5 mL of the FeSSIF Dissolving Buffer was then added 1 mL stepwise forming an emulsion. The solution was transferred to a 50-mL volumetric flask and diluted to volume with the FeSSIF Dissolving Buffer.

An alternative media (“FeSSIF alternative media”) was used to study the trifluoroacetate salts (Type A and B) and the HCl Type I salt. More specifically, the media was prepared by transferring 0.41 mL of glacial acid and weighing 0.20 g of sodium hydroxide, 0.59 g of sodium chloride into a 50-mL volumetric flask. This was dissolved with approximately 48 mL of purified water. The pH was adjusted to exactly 5.0 using 1 M HCl or 1 M NaOH and diluted to volume with purified water. 0.56 g of SIF powder was added, stirred and sonicated until all the powder is completely dissolved. The solution can be stored at RT for 48 hours or 4° C. for 7 days and should be equilibrated to RT before use.

Dextrose Buffer (pH 5.5) Preparation—The terms “dextrose buffer at pH 5.5” and “dextrose buffer (pH 5.5)” mean a solution prepared according to the following method. Dextrose (0.5 g) was added to a 100-mL volumetric flask. Then 1M HCl or 1M NaOH was added to adjust the pH of the buffer to pH 5.5.

Acetate Buffer (pH 5.5) Preparation—The acetate buffer (pH 5.5) used for the trifluoroacetate salts (Type A and B) and the HCl Type I salt was prepared by placing 0.60 g sodium acetate (NaC₂H₃O₂.3H₂O) in a 100-mL volumetric flask, adding 3 mL of 2 M acetic acid solution, and then adding purified water to volume.

Phosphate Buffer (pH 6.0) Preparation—The terms “phosphate buffer at pH 6.0” and “phosphate buffer (pH 6.0)” mean a solution prepared according to the following method. A solution of 0.2 M KH₂PO₄ (25 mL) and 0.2 M NaOH (5.6 mL) was prepared in a 100-mL volumetric flask. The pH was checked by pH meter. Then water was added to volume.

An alternative media (“phosphate (pH 6.0) alternative media”) was used to study the trifluoroacetate salts (Type A and B) and the HCl Type I salt. More specifically, the media was prepared by dissolving 2.72 g of 0.2 M monobasic potassium phosphate (KH₂PO₄) in purified water, and diluting with purified water to 100 mL. 0.8 g of 0.2 M sodium hydroxide in purified water was diluted with purified water to 100 mL. Then 50 mL of the 0.2 M monobasic potassium phosphate solution was placed in a 200-mL volumetric flask, 5.6 mL of 0.2 M sodium hydroxide solution was added, and then purified water was added to volume.

Phosphate Buffer (pH 7.5) Preparation—The terms “phosphate buffer at pH 7.5” and “phosphate buffer (pH 7.5)”mean a solution prepared according to the following method. A solution of 0.2 M KH₂PO₄ (25 mL) and 0.2 M NaOH (40.2 mL) was prepared in a 100-mL volumetric flask. The pH was checked by pH meter. Then water was added to volume.

Kinetic Solubility of SCY-078 Salts—The term “kinetic solubility” with respect to SCY-078 salts means the following procedure. First, 15 mg, 50 mg, or 100 mg of one of the SCY-078 salts was placed into a 4-mL plastic centrifuge tubes along with 1.7 mL of relevant media or 2.0 mL of water. For dextrose buffer at pH 5.5, phosphate buffer at pH 6.0, and phosphate buffer at pH 7.5, 15 mg of the SCY-078 salt was used. For SGF media, FeSSIF media, and FaSSIF media, 50 mg of the SCY-078 salt was used. For water, 100 mg of the SCY-078 salt was used. The actual weight of each sample was recorded. The tube was subsequently capped and the suspension samples were stirred on a rolling incubator (25 rpm) at room temperature. Samples were taken at 1 hour, 4 hours, and 24 hours respectively. For each sample, a 0.5 mL aliquot of the suspension was transferred into a 1.5-mL centrifuge filtration tube and centrifuged. The samples were then filtered through the centrifuge filtration tube (0.45 μm) at 8,000 rpm at room temperature for 3 minutes.

The trifluoroacetate salts (Type A and B) and the HCl Type I salt were tested using the following alternative procedure. First, 15 mg, 36 mg or 90 mg solid was weighted into a 4-mL plastic tube, and 3 mL of relevant media was added before leaving the suspension on a rolling incubator (25 r/min). For SCF, 90 mg of solid was used. For FaSSIF, acetate buffer (pH 5.5), and phosphate buffer (pH 6.0), 15 mg solid was used. For FeSSIF, 36 mg of solid was used. 1.0 mL aliquot of the suspension was sampled for centrifugation with the supernatant submitted for HPLC and pH measurement and solid for XRPD characterization at 1 hr, 4 hr and/or 24 hrs.

Approximate solubility of SCY-078 salts—The term “approximate solubility” with respect to SCY-078 salts means the procedure described in this paragraph. To conduct each experiment, a sample of a SCY-078 salt (˜2 mg) was added into a 3-mL glass vial. Then a solvent was added step-wise (100 μL per step) into the vials until the solids were dissolved or a total volume of 2 mL was reached.

Equilibrium solubility of SCY-078 salts—The term “equilibrium solubility” with respect to SCY-078 salts means the procedure described in this paragraph. The equilibrium solubility of a SCY-078 salt was evaluated in water at room temperature. First, the SCY-078 salt (˜50 mg) was weighed into a 1.5-mL vial followed by addition of 1.0 mL water, and then the sample was stirred (800 rpm) at room temperature for 24 hours. The sample was centrifuged with the residual solid analyzed by XRPD and supernatant concentration measured by HPLC.

Polarized light microscopic imaging—Polarized light microscopic (PLM) images was captured at room temperature using Axio Lab A1 upright microscope equipped with ProgRes® CT3 camera. The sample was sandwiched between a glass slide and a top cover before placed under the polarized light microscopy for imaging.

Example 1

SCY-078 Phosphate: The phosphate salt of SCY-078 was prepared from SCY-078 freebase, which was prepared using known procedures. See, e.g., U.S. Pat. No. 8,188,085. SCY-078 freebase (10.0 g) was placed in a 250 mL reactor. Ethanol (50 mL), ethyl acetate (30 mL), acetic acid (1.5 mL) and water (1 mL) were added and the mixture was stirred at room temperature over 10 minutes. The resulting homogeneous solution was heated to 50° C. and phosphoric acid (1.74 g) solution in ethyl acetate was slowly added to the solution at 50° C. for 1 hour. The resulting slurry was slowly cooled to room temperature and stirred overnight at room temperature. The slurry was filtered, and the wet cake was washed with 20 mL mixed solvents (ethanol:ethyl acetate:water=5:5:0.1) two times, then twice with ethyl acetate (1 mL). The wet cake was dried under vacuum with nitrogen sweep over three hours, and then dried in a vacuum oven overnight to obtain 11.08 g of an off-white crystal. The retention time of the compound was 4.08 minutes, as measured by HPLC using an Ascentis Express C18 column with standard gradient: 10-95% of B in 6 minutes (A=0.1% phosphoric acid, B=acetonitrile), 2 minute hold 2 minute post; flow rate: 1.8mL/minute (UV detection at 245 nm, 40° C.).

SCY-078 phosphate was characterized by XRPD, which evidenced that the compound is crystalline (FIG. 1). The 2 theta and d-spacing values are summarized in Table 4. The DSC curve of SCY-078 phosphate exhibited two endothermic peaks at 48.1° C. and 267° C. (FIG. 2). A weight loss of 6.6% was observed up to 155.4° C. in the TGA curve (FIG. 2).

TABLE 4 FWHM Left Pos. [°2Th.] Height [cts] [°2Th.] d-spacing [Å] Rel. Int. [%] 3.446579 121.838400 0.153504 25.63591 8.36 6.002339 410.263500 0.127920 14.72481 28.14 6.926787 321.574500 0.153504 12.76157 22.05 7.923199 635.487400 0.179088 11.15879 43.58 9.163023 1458.149000 0.230256 9.65152 100.00 9.949172 420.112200 0.153504 8.89059 28.81 10.395180 355.706200 0.204672 8.51011 24.39 11.606700 1098.847000 0.179088 7.62440 75.36 11.998390 742.370400 0.153504 7.37637 50.91 12.509650 1152.457000 0.230256 7.07603 79.04 14.390130 1325.262000 0.179088 6.15529 90.89 15.561700 1295.270000 0.230256 5.69443 88.83 16.742160 847.559600 0.230256 5.29549 58.13 17.427190 694.495600 0.179088 5.08885 47.63 18.989620 584.788500 0.204672 4.67353 40.10 19.700330 472.891400 0.204672 4.50650 32.43 20.641180 788.029700 0.153504 4.30316 54.04 22.864060 331.310800 0.204672 3.88959 22.72 24.026930 187.756700 0.307008 3.70391 12.88 25.365620 208.707200 0.358176 3.51139 14.31 28.405150 77.468630 0.614016 3.14218 5.31 30.814330 70.380370 0.818688 2.90179 4.83

Example 2

SCY-078 Crystalline Freebase (MeOH desolvate): The MeOH desolvate was prepared as follows. SCY-078 phosphate salt (10.0 g) was charged into a 250 mL reactor. Sodium carbonate (50 mL of a 10% solution) was added at 20° C. and agitated. 2-methyltetrahydrofuan (100 mL) was added and agitated strongly at 20° C. until all the solids dissolved. The mixture was left to stand for 30 minutes to leave two clear layers which were separated and the organic layer was washed twice with deionized water (40 mL). The washed organic layer was transferred to a 125-mL reaction vessel and agitated at 500 rpm, heated to 50° C. and distilled under partial vacuum at 50° C. down to 40 mL volume. Methanol (80 mL) was added to the reaction vessel at 50° C., which was then cooled to 40° C.; after 2 hours, crystals formed. The volume was then distilled down to 50 mL at 40° C. under partial vacuum over 16 hours. There was then constant volume distillation at 40° C. while adding methanol (40 mL) over 2 hours. Water (20 mL) was then added over 2 hours. The reaction vessel was then cooled to 20° C. over 2 hours and then slurry aged at 20° C. for 2 hours. The mixture was then filtered and the resulting wet cake washed with 20 mL of a 4:1 solution of methanol and water. The wet cake was dried under nitrogen sweep at room temperature for 16 hours. XRPD analysis confirmed that the dry cake is desolvated methanol solvate (yield 89%, purity: 99.1%).

Two batches of MeOH desolvate were prepared and characterized by XRPD, DSC, and TGA (FIGS. 3-5). XRPD patterns evidenced that the compound is crystalline. The 2 theta and d-spacing values from Batch 1 and Batch 2 are summarized in Tables 5a and 5b, respectively. The DSC curve of MeOH desolvate Batch 1 exhibited an endotherm at ˜55.0° C. and an exotherm at ˜281.5° C. The DSC curve of MeOH desolvate Batch 2 exhibited an endotherm at ˜56.1° C. and an exotherm at ˜279.2° C. The TGA curve of Batch 1 showed a weight loss of 4.7% before 120° C. The TGA curve of Batch 1 showed a weight loss of 6.6% before 120° C. The TGA curve of Batch 2 showed a weight loss of 4.9% before 120° C.

TABLE 5a FWHM Left Pos. [°2Th.] Height [cts] [°2Th.] d-spacing [Å] Rel. Int. [%] 6.763088 656.275900 0.153504 13.07009 7.96 7.007256 762.055000 0.127920 12.61520 9.24 7.747366 5938.858000 0.179088 11.41165 72.02 8.156786 1751.303000 0.179088 10.83975 21.24 9.712988 1115.822000 0.153504 9.10623 13.53 10.141860 2106.808000 0.076752 8.72210 25.55 12.584090 950.310500 0.153504 7.03433 11.52 13.014360 1356.119000 0.127920 6.80272 16.45 13.486330 1749.145000 0.102336 6.56570 21.21 13.729600 2433.802000 0.076752 6.44991 29.52 14.005940 8245.783000 0.166296 6.32326 100.00 14.405120 4246.837000 0.140712 6.14892 51.50 14.876760 4956.689000 0.127920 5.95503 60.11 15.210400 2019.649000 0.089544 5.82515 24.49 15.545740 4247.989000 0.153504 5.70024 51.52 15.924930 1123.799000 0.127920 5.56535 13.63 16.357440 911.992900 0.102336 5.41916 11.06 16.653970 1230.506000 0.153504 5.32333 14.92 17.281780 1476.632000 0.115128 5.13134 17.91 18.290550 2584.896000 0.127920 4.85054 31.35 18.910970 2762.926000 0.089544 4.69279 33.51 19.423650 3792.983000 0.166296 4.57006 46.00 20.154410 631.061700 0.153504 4.40598 7.65 21.211700 1085.950000 0.153504 4.18870 13.17 22.103930 642.381300 0.153504 4.02160 7.79 22.813500 357.089200 0.153504 3.89809 4.33 23.851840 485.307900 0.204672 3.73070 5.89 24.372350 254.224300 0.153504 3.65219 3.08 25.127990 631.886600 0.204672 3.54405 7.66 25.582260 817.763400 0.230256 3.48214 9.92 25.946780 361.733700 0.127920 3.43404 4.39 27.215450 399.894700 0.204672 3.27677 4.85 31.598700 117.418900 0.358176 2.83152 1.42 34.043420 360.328100 0.179088 2.63357 4.37 35.453150 228.142700 0.204672 2.53202 2.77 37.397760 89.968660 0.204672 2.40471 1.09

TABLE 5b FWHM Left Pos. [°2Th.] Height [cts] [°2Th.] d-spacing [Å] Rel. Int. [%] 6.689568 495.508900 0.076752 13.21357 7.10 6.958760 795.039300 0.076752 12.70301 11.39 7.719929 6977.127000 0.102336 11.45214 100.00 8.113519 1569.148000 0.076752 10.89746 22.49 9.660554 883.585800 0.089544 9.15554 12.66 10.105090 1377.370000 0.063960 8.75376 19.74 12.511740 551.167400 0.076752 7.07485 7.90 12.972470 906.794200 0.102336 6.82459 13.00 13.432450 898.612700 0.051168 6.59191 12.88 13.698610 1280.818000 0.063960 6.46442 18.36 13.963580 6943.854000 0.102336 6.34235 99.52 14.354450 3936.292000 0.102336 6.17051 56.42 14.833880 3242.652000 0.102336 5.97215 46.48 15.161830 1072.948000 0.102336 5.84370 15.38 15.499310 3840.597000 0.115128 5.71721 55.05 15.870270 694.539900 0.063960 5.58440 9.95 16.294450 561.885100 0.102336 5.43997 8.05 16.589520 716.170000 0.076752 5.34387 10.26 17.231620 1048.768000 0.089544 5.14616 15.03 18.228150 1455.529000 0.089544 4.86701 20.86 18.853630 2320.288000 0.102336 4.70693 33.26 19.358690 2769.789000 0.127920 4.58525 39.70 20.105050 319.040400 0.153504 4.41669 4.57 21.158160 854.556900 0.089544 4.19918 12.25 22.060990 457.091300 0.127920 4.02933 6.55 22.755630 189.699300 0.153504 3.90788 2.72 23.818130 259.770000 0.102336 3.73590 3.72 24.629730 250.065300 0.153504 3.61461 3.58 25.034030 415.901700 0.102336 3.55714 5.96 25.544070 535.863500 0.179088 3.48726 7.68 25.883800 303.412800 0.102336 3.44225 4.35 27.200370 321.682800 0.102336 3.27856 4.61 27.597540 179.014900 0.102336 3.23227 2.57 28.068430 126.035900 0.409344 3.17911 1.81 29.974950 124.045000 0.153504 2.98110 1.78 31.402190 89.293980 0.614016 2.84879 1.28 32.603460 118.588300 0.204672 2.74652 1.70 33.988800 247.854700 0.179088 2.63768 3.55 35.391040 161.830400 0.153504 2.53632 2.32

Example 3

SCY-078 Amorphous Freebase: To prepare SCY-078 amorphous freebase, MeOH desolvate (50 mg) was added to a 3-mL vial. Then DCM (0.5 mL) was added to the vial of MeOH desolvate. The resulting solution of MeOH desolvate and DCM formed a clear solution. The solution was evaporated to dryness from an open vial at 50° C.

The solid obtained after evaporation was characterized by XRPD, TGA, DSC, and DVS. The XRPD pattern evidenced that the tested sample is amorphous. The DSC and TGA curves of the amorphous sample exhibited a glass transition at ˜189.1° C. (FIG. 6). The TGA curve demonstrated a weight loss of 4.2% before 150° C. The DVS curve demonstrated that the sample is hygroscopic with a water uptake of ˜4.8% at 80% RH, 25° C. An XRPD pattern performed after DVS demonstrated no form change.

Example 4

Kinetic Solubility of SCY-078 Freebase: The kinetic solubility of SCY-078 MeOH desolvate and SCY-078 amorphous freebase was evaluated in SGF media, FaSSIF media, FeSSIF media, dextrose buffer (pH 5.5), phosphate buffer (pH 6.0), and phosphate buffer (pH 7.5) at room temperature. First, solid SCY-078 MeOH desolvate or SCY-078 amorphous freebase (˜15 mg) was weighed into a 4-mL vial. Then the relevant media (3.0 mL) was added and the suspensions were stirred on a rolling incubator (25 rpm) at room temperature for 1 hour, 4 hours, and 24 hours respectively. After stirring, 0.5 mL of suspension was centrifuged and filtered (0.45 μm). The residual solids were analyzed by XRPD, and the supernatant was measured by HPLC and pH meter.

The results (Table 6) suggested that both the MeOH desolvate and the amorphous freebase display high solubility in SGF and FeSSIF. The results also showed that both the MeOH desolvate and the amorphous freebase are only sparingly soluble in FaSSIF and pH 5.5, pH 6.0, and pH 7.5 buffers.

Solid form change was observed during the solubility measurements of the MeOH desolvate in FeSSIF, FaSSIF, pH 5.5 and 6.0 buffers. Additionally, three new crystal forms were discovered (Table 6). The three new forms are identified as New Form 1, 2, and 3.

TABLE 6 Kinetic solubility of SCY-078 freebase Starting Time Solubility Solvent Form (hours) (mg/mL) pH Form SGF Desolv.^($) 1 >5.3* 2.8 N/A 4 >5.3* 2.8 N/A 24 >5.3* 2.8 N/A Amorph. 1 >5.2* 2.1 N/A 4 >5.2* 2.2 N/A 24 >5.2* 2.2 N/A FeSSIF Desolv. 1 3.3 5.1 NF 1 4 3.5 5.1 NF 1 24 3.5 5.1 NF 1 Amorph. 1 3.0 5.1 Amorph. 4 3.7 5.1 Amorph. 24 3.8 5.1 Amorph. FaSSIF Desolv. 1 ND 6.6 NF 1 4 ND 6.6 NF 1 24 ND 6.6 NF 1 Amorph. 1 0.017 6.6 Amorph. 4 <0.51 μg/mL 6.6 Amorph. 24 ND 6.6 Amorph. pH 5.5 Desolv. 1 N/A N/A N/A buffer 4 0.0008 7.2 NF 2 24 ND 7.1 NF 2 Amorph. 1 N/A N/A N/A 4 ND 6.8 Amorph. 24 ND 6.9 Amorph. pH 6.0 Desolv. 1 N/A N/A N/A buffer 4 0.045 6.2 NF 1 24 0.60 6.1 NF 3 Amorph. 1 N/A N/A N/A 4 <0.51 μg/mL 6.1 Amorph. 24 0.0024 6.1 Amorph, pH 7.5 Desolv. 1 N/A N/A N/A buffer 4 0.0024 7.3 Desolv. 24 0.0025 7.4 Desolv. Amorph. 1 N/A N/A N/A 4 ND 7.4 Amorph. 24 ND 7.4 Amorph. *Clear solution was obtained NF: New Form ND: Not Detected ^($)MeOH desolvate N/A: Not available Desolv.: Desolvate Amorph.: Amorphous

Example 5

Approximate Solubility of SCY-078 MeOH Desolvate: The approximate solubility of SCY-078 MeOH desolvate was measured in 20 solvents at room temperature (25±3° C.). First, MeOH desolvate (˜2 mg) was added to a 3-mL glass vial. Then the corresponding solvent was added step wise (100 μL) until the solution was visually clear or a total volume of 2 mL was reached. The results appear in Table 7.

TABLE 7 Approximate solubility of SCY-078 freebase at RT Solvent Solubility (mg/mL) MeOH 5.4 < S < 6.3 EtOH 2.6 < S < 3.0 IPA 7.0 < S < 11.0 Acetic Acid S >25.0 ACN 2.6 < S < 2.9 Acetone 8.3 < S < 12.5 MIBK S >29.0 EtOAc S >23.0 iPrOAc 10.5 < S < 21.0 MTBE S >23.0 THF S >23.0 2-MeTHF S >25.0 1,4-Dioxane S >37.0 NMP S >45.0 DMSO S >32.0 CHCl₃ S >33.0 Toluene 6.8 < S < 8.5 Heptane S <2.2 DMA S >40.0 H₂O S <1.9

Example 6

Salt Study of SCY-078 Freebase: A salt study of the SCY-078 MeOH desolvate freebase was performed using 108 different conditions developed through 18 acids in 6 solvents (Table 8). The salt study was performed by first preparing a solution of SCY-078 MeOH desolvate freebase and mixing with an equi-molar acid solution. This solution was stirred at room temperature overnight.

For precipitates, the solids were isolated and analyzed by XRPD. Clear solutions were evaporated slowly to dryness at room temperature.

The salt study (Table 8) showed that seven crystalline salts (eight crystal forms) of SCY-078 were found: HCl Type A, citrate Type A, hippurate Type A, fumarate Type A, fumarate Type B, glycolate Type A, mesylate Type A, and Ca salt Type A. Four crystal forms of SCY-078 freebase were discovered during the salt study and were identified as freebase (“FB”) Type A, B, C, and D.

TABLE 8 Solvent THF/H₂O Acid EtOH (A) IPA (B) ACN (C) Acetone (D) EtOAc (E) (19:1, v/v) (F) HCl oil oil HCl salt HCl salt amorphous oil Type A Type A H₂PO₄ oil oil oil oil oil oil Maleic Acid amorphous amorphous FB amorphous amorphous amorphous Type A Citric Acid amorphous amorphous Citrate amorphous FB amorphous Type A Type A Hippuric Acid FB FB Hippurate Hippurate Hippurate Low Type B Type B Type A Type A Type A crystallinity Adipic Acid amorphous FB FB FB amorphous amorphous Type A Type A Type C Fumaric Acid amorphous amorphous Fumarate FB Fumarate amorphous Type A Type D Type B Glutaric Acid amorphous FB FB FB FB amorphous Type A Type A Type C Type A Glycolic Acid FB FB FB FB FB Glycolate Type B Type B Type B Type C Type B Type A D-Glutamic MeOH FB FB FB FB amorphous Acid desolvate + acid Type A Type A Type C Type A Acetic Acid oil oil amorphous amorphous amorphous oil Mucic Acid amorphous amorphous FB FB FB amorphous Type D Type D Type D L-Malic Acid amorphous amorphous FB FB FB amorphous Type A Type C Type A Benzoic acid MeOH FB FB FB FB amorphous Desolvate Type A Type A Type C Type A Methanesulfonic amorphous amorphous FB Mesylate FB amorphous Acid Type B Type A Type B Malonic Acid amorphous amorphous FB amorphous FB amorphous Type A Type A Ethanesulfonic amorphous oil FB FB FB amorphous Acid Type B Type B Type B Ca(OH)₂ Ca(OH)₂ Ca(OH)₂ FB FB Ca(OH)₂ Ca salt Type D Type C Type A All the above salt formation experiments were performed at RT using 1.0 mole equivalency of acid. FB: freebase

Example 7

SCY-078 HCl Type A: SCY-078 HCl Type A prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 7-8). The resulting XRPD pattern evidenced that SCY-078 HCl Type A is weakly crystalline and has a unique form as compared to the freebase MeOH desolvate. The 2 theta and d-spacing values are summarized in Table 9. The DSC curve displayed an endotherm at 48.5° C. (onset temperature). The TGA curve showed a weight loss of 14.4% before 130° C.

TABLE 9 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 5.635436 167.080400 0.153504 15.68264 21.28 9.023307 62.106750 0.307008 9.80065 7.91 11.313240 785.111500 0.179088 7.82150 100.00 14.217720 329.207600 0.179088 6.22955 41.93 16.983670 169.700900 0.204672 5.22073 21.61 18.224350 96.247500 0.409344 4.86801 12.26 32.709530 91.332090 0.153504 2.73786 11.63

Example 8

SCY-078 Citrate Type A (Molar Equivalency—counter ion/API 1): SCY-078 citrate Type A prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 9-10). The resulting XRPD pattern evidenced that SCY-078 citrate Type A is crystalline and is a unique form compared to the freebase MeOH desolvate. The 2 theta and d-spacing values are summarized in Table 10. The DSC curve displayed two endotherms at 36.2° C. and 194.8° C. (onset temperature). The TGA curve demonstrated 5.1% before 100° C.

A sample was heated to 100° C. and then cooled to room temperature. XRPD was performed after heating and cooling to room temperature. The resulting XRPD pattern showed that there was no change in form. DSC and TGA characterization was also performed after heating and cooling. The DSC curve demonstrated two endotherms at 39.9° C. and 194.8° C. (onset temperatures). The TGA curve showed a weight loss of 5.3% before 100° C.

TABLE 10 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 5.448309 886.677500 0.140712 16.22084 29.56 7.551316 2999.844000 0.153504 11.70747 100.00 9.280401 338.350200 0.204672 9.52971 11.28 10.933390 532.481900 0.102336 8.09237 17.75 11.525650 1003.802000 0.140712 7.67783 33.46 12.550580 416.868300 0.204672 7.05304 13.90 13.236060 1150.251000 0.153504 6.68928 38.34 15.063710 476.465200 0.153504 5.88154 15.88 16.766510 1157.775000 0.204672 5.28786 38.59 18.032270 212.464400 0.153504 4.91943 7.08 19.686700 214.063600 0.307008 4.50959 7.14 22.220460 123.975800 0.153504 4.00077 4.13 30.421720 65.589230 0.358176 2.93833 2.19 34.915250 28.764450 0.614016 2.56979 0.96

Example 9

SCY-078 Hippurate Type A (Molar equivalency counter ion/API 2.0): SCY-078 hippurate Type A prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 11-12). The XRPD pattern of SCY-078 hippurate Type A indicated that the sample is crystalline and has a unique form as compared to the freebase MeOH desolvate. The DSC curve displayed three endotherms at 36.3° C., 104.6° C., and 165.5° C. (onset temperatures) and one exotherm at 201.9° C. (onset temperature). The TGA curve showed a weight loss of 4.9% before 150° C.

Example 10

SCY-078 Hippurate Type B: SCY-078 hippurate Type B is produced from heating hippurate Type A to 150° C. and then cooling the sample to room temperature. SCY-078 hippurate Type B was characterized by XRPD, DSC, and TGA (FIGS. 13-14). The XRPD pattern showed that the sample is crystalline and a unique form compared to SCY-078 hippurate Type A. The DSC curve displayed two endotherms at 39.6° C. and 166.4° C. (onset temperatures) and one exotherm at 201.4° C. (onset temperature). The TGA curve demonstrated a weight loss of 1.8% before 150° C.

Example 11

SCY-078 Hippurate Type C: Cycle DSC and XRPD were performed to investigate the phase transition events during the heating-cooling process of SCY-078 hippurate Type B (FIGS. 15-16). The XRPD overlay and DSC curve suggested the melting point of SCY-078 hippurate Type B at 163.9° C. followed by amorphous phase recrystallizing at 208.8° C. and a new anhydrate phase being formed. The new anhydrate phase is SCY-078 hippurate Type C.

Example 12

SCY-078 Fumarate Type A (Molar equivalency counter ion/API 1.0): SCY-078 fumarate Type A prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 17-18). The XRPD pattern indicated that the sample is crystalline and a unique form compared to the freebase MeOH desolvate. The DSC curve of SCY-078 fumarate Type A showed an endotherm at 33.1° C. and a melting point at 207.3° C. (onset temperature). The TGA curve displayed a weight loss of 2.4% before 120° C.

A sample of SCY-078 fumarate Type A was heated to 120° C. and then allowed to cool to room temperature. Characterization by XRPD, DSC, and TGA were then repeated. The XRPD pattern displayed no form change after heating and cooling. The DSC curve of heated-cooled SCY-078 fumarate Type A exhibited two endotherms at 38.4° C. and 207.1° C. (onset temperatures). The TGA curve of heated-cooled SCY-078 fumarate Type A showed a weight loss of 2.0% before 120° C.

Example 13

SCY-078 Fumarate Type B (Molar equivalency counter ion/API 0.8): SCY-078 fumarate Type B prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 19-20). The XRPD pattern of SCY-078 fumarate Type B indicated that the sample is weakly crystalline and that it is a unique form compared to the freebase MeOH desolvate. The DSC curve of SCY-078 fumarate Type B showed two endotherms at 37.9° C. and 178.5° C. (onset temperature). The TGA curve demonstrated a weight loss of 13.4% before 300° C.

Example 14

SCY-078 Glycolate Type A (Molar equivalency counter ion/API 2.0): SCY-078 glycolate Type A prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 21-22). The XRPD pattern of SCY-078 glycolate Type A indicated that the sample is crystalline and a unique form compared to the freebase MeOH solvate. The DSC curve of the sample displayed two endotherms at 35.9° C. and 159.6° C. (onset temperatures). The TGA curve showed a weight loss of 6.6% before 100° C.

Example 15

SCY-078 Mesylate Type A (Molar equivalency counter ion/API 1.0): SCY-078 mesylate Type A prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 23-24). The XRPD pattern indicated that the sample is crystalline and a unique form compared to the freebase MeOH desolvate. The DSC curve displayed an endotherm at 44.2° C. and a melting point at 260.0° C. (onset temperatures). The TGA showed a weight loss of 4.5% before 120° C.

Next, a sample of SCY-078 mesylate Type A was heated to 120° C. and then allowed to cool to room temperature. Characterization by XRPD, DSC, and TGA was then repeated. The XRPD pattern displayed no form change after heating and cooling. The DSC curve of the heated-cooled SCY-078 mesylate Type A exhibited an endotherm at 59.7° C. and a melting point at 257.4° C. (onset temperatures). The TGA curve of the heated-cooled SCY-078 mesylate Type A showed a weight loss of 9.4% before 120° C.

Example 16

SCY-078 Calcium Type A: SCY-078 calcium Type A prepared from the salt study in Example 6 was characterized by XRPD, DSC, and TGA (FIGS. 25-26). The XRPD pattern indicated that the sample is crystalline and a unique form compared to the freebase MeOH desolvate. The DSC curve displayed two endotherms at 147.3° C. and 230.8° C. (onset temperatures). The TGA curve showed a weight loss of 5.3% before 170° C.

Example 17

Preparation and Characterization of Scaled-Up SCY-078 Hippurate Type B: To scale-up SCY-078 hippurate Type B, a solution of hippuric acid (122.8 mg) and SCY-078 MeOH desolvate (500.3 mg) in ACN (5.0 mL) was prepared. The suspension was then stirred (500 rpm) at room temperature for 28 hours. Following stirring, some slurry was filtered and the isolated solid was checked by XRPD to confirm SCY-078 hippurate Type A. The suspension was filtered and dried at 150° C. for 1 hour before characterization. Finally, the solid was check by XRPD to confirm to SCY-078 hippurate Type B.

The scaled-up SCY-078 hippurate Type B was analyzed by XRPD, DSC, TGA, and DVS (FIGS. 27-30). The resulting XRPD pattern evidenced that SCY-078 hippurate Type B was successfully scaled up. The 2 theta and d-spacing values are summarized in Table 11. The DSC curve showed two endotherms at 34.5° C. and 164.4° C. and one exotherm at 205.2° C. (onset temperatures). The TGA curve showed a weight loss of 0.9% before 130° C. The DVS curve showed that the sample is hygroscopic with a water uptake of -3.5% at 25° C. and 80% RH. A second XRPD pattern performed after DVS showed SCY-078 hippurate Type B converted to SCY-078 hippurate Type A after DVS experiment.

TABLE 11 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 3.027072 11818.150000 0.051168 29.18766 100.00 5.916137 351.270000 0.102336 14.93916 2.97 6.916698 947.910600 0.102336 12.78016 8.02 7.251646 624.847700 0.076752 12.19059 5.29 8.761702 1828.733000 0.076752 10.09268 15.47 9.962105 2404.236000 0.102336 8.87907 20.34 10.897800 1593.408000 0.102336 8.11872 13.48 11.868550 552.254500 0.102336 7.45677 4.67 12.432300 2880.441000 0.127920 7.11988 24.37 12.857840 525.634600 0.076752 6.88518 4.45 13.091360 511.764400 0.115128 6.76288 4.33 13.709840 1112.219000 0.102336 6.45916 9.41 14.555290 3086.294000 0.153504 6.08582 26.11 14.984610 1215.693000 0.102336 5.91241 10.29 15.341160 506.870800 0.153504 5.77579 4.29 16.136210 1315.742000 0.089544 5.49296 11.13 16.453540 1710.358000 0.153504 5.38772 14.47 16.897030 606.324900 0.102336 5.24730 5.13 17.280760 1171.798000 0.127920 5.13164 9.92 17.591700 2258.867000 0.102336 5.04163 19.11 18.190770 538.754800 0.127920 4.87692 4.56 18.425670 516.831300 0.179088 4.81528 4.37 19.151570 950.084500 0.102336 4.63437 8.04 19.602330 487.956400 0.127920 4.52881 4.13 20.234760 861.917600 0.153504 4.38867 7.29 20.860030 424.598600 0.153504 4.25851 3.59 21.725360 459.496200 0.307008 4.09081 3.89 22.532320 498.240700 0.102336 3.94610 4.22 23.078810 380.947900 0.127920 3.85388 3.22 23.551950 208.488500 0.409344 3.77752 1.76 23.874020 377.598600 0.102336 3.72728 3.20 25.381750 351.553600 0.102336 3.50919 2.97 25.844490 207.070300 0.204672 3.44740 1.75 27.188450 192.463400 0.153504 3.27997 1.63 27.681830 144.369000 0.307008 3.22262 1.22 29.319670 172.870900 0.511680 3.04622 1.46 30.833510 86.432220 0.307008 2.90002 0.73 34.979000 90.330020 0.204672 2.56525 0.76 35.588330 69.479680 0.307008 2.52271 0.59 37.270360 55.666410 0.307008 2.41264 0.47

Example 18

Preparation and Characterization of Scaled-Up SCY-078 Fumarate Type A: To scale-up SCY-078 fumarate Type A, a solution of fumaric acid (79.8 mg) and SCY-078 MeOH desolvate (501.9 mg) in ACN (15.0 mL) was prepared. The suspension was then stirred (500 rpm) at room temperature for 28 hours. Following stirring, some slurry was filtered and the isolated solid was checked by XRPD to confirm SCY-078 fumarate Type A. Finally, the suspension was filtered and dried at 30° C. for 4 hours in vacuum before characterization.

Characterization of scaled-up SCY-078 fumarate Type A included XRPD, DSC, TGA, and DVS (FIGS. 31-33). The resulting XRPD pattern evidenced that SCY-078 fumarate Type A was successfully scaled up. The 2 theta and d-spacing values are summarized in Table 12. The DSC curve showed an endotherm at 39.9° C. and a melting endotherm at 208.4° C. (onset temperatures). The TGA curve showed a weight loss of 1.7% before 150° C. The DVS curve showed that the sample is hygroscopic with a water uptake of 2.5% at 80% RH, 25° C. A second XRPD pattern performed after DVS showed SCY-078 fumarate Type A had no form change.

TABLE 12 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 6.708362 290.801200 0.153504 13.17659 2.14 7.779830 2540.992000 0.102336 11.36410 18.74 8.490587 13558.710000 0.127920 10.41433 100.00 9.362659 1986.519000 0.102336 9.44617 14.65 9.964151 661.033600 0.127920 8.87725 4.88 10.417770 2882.948000 0.115128 8.49171 21.26 10.694630 3478.282000 0.127920 8.27250 25.65 11.221780 3785.363000 0.140712 7.88504 27.92 11.875520 519.613200 0.153504 7.45241 3.83 12.271310 357.834700 0.127920 7.21292 2.64 13.008060 5831.607000 0.140712 6.80600 43.01 13.729940 1780.661000 0.127920 6.44975 13.13 14.640000 1169.640000 0.076752 6.05080 8.63 14.888350 2319.347000 0.078000 5.94549 17.11 14.955620 2471.802000 0.076752 5.92381 18.23 15.225230 1608.774000 0.089544 5.81951 11.87 16.171470 1276.652000 0.076752 5.48106 9.42 16.429100 2604.447000 0.140712 5.39569 19.21 16.942700 6000.498000 0.140712 5.23326 44.26 17.442280 1878.502000 0.063960 5.08448 13.85 17.749440 3552.116000 0.140712 4.99718 26.20 18.202980 2297.875000 0.127920 4.87368 16.95 18.928920 1597.690000 0.140712 4.68838 11.78 20.028870 591.031400 0.127920 4.43332 4.36 20.258840 480.204800 0.102336 4.38351 3.54 20.868380 1498.769000 0.127920 4.25683 11.05 21.397940 1555.987000 0.089544 4.15266 11.48 21.879750 1130.218000 0.127920 4.06229 8.34 22.217300 1475.131000 0.102336 4.00133 10.88 22.591130 913.600300 0.102336 3.93596 6.74 23.385000 425.684600 0.153504 3.80411 3.14 23.950880 915.135300 0.204672 3.71550 6.75 24.627720 245.047100 0.153504 3.61490 1.81 25.499420 1445.187000 0.089544 3.49326 10.66 26.148510 509.814300 0.179088 3.40800 3.76 26.502880 512.161300 0.127920 3.36323 3.78 26.881710 614.926800 0.153504 3.31669 4.54 27.716900 269.647600 0.255840 3.21862 1.99 28.552140 414.549300 0.153504 3.12634 3.06 29.217330 471.259100 0.153504 3.05666 3.48 29.558860 525.910500 0.204672 3.02211 3.88 30.043740 270.781000 0.204672 2.97444 2.00 30.962340 570.115500 0.307008 2.88825 4.20 33.133860 264.449500 0.179088 2.70376 1.95 33.967430 212.730600 0.179088 2.63929 1.57 34.456190 314.358700 0.179088 2.60297 2.32 35.316070 163.009400 0.179088 2.54153 1.20 35.928310 161.442300 0.307008 2.49962 1.19 37.556590 162.885200 0.153504 2.39491 1.20

Example 19

Preparation and Characterization of Scaled-Up SCY-078 Mesylate Type A: To scale up SCY-078 mesylate Type A, a solution of methanesulfonic acid (66.7 mg) and SCY-078 MeOH desolvate (500.00 mg) in ACN (6.0 mL) was prepared. The suspension was then stirred (500 rpm) at room temperature for 28 hours. Following stirring, some slurry was filtered and the isolated solid was checked by XRPD to confirm to SCY-078 mesylate Type A. Finally, the suspension was filtered and dried at 30° C. for 4 hours in vacuum before characterization.

Characterization of scaled-up SCY-078 mesylate Type A included XRPD, DSC, TGA, and DVS (FIGS. 34-36). The resulting XRPD pattern evidenced that SCY-078 mesylate Type A was successfully scaled up. The 2 theta and d-spacing values are summarized in Table 13. The DSC curve showed an endotherm at 45.1° C. and a melting endotherm at 252.5° C. (onset temperatures). The TGA curve showed a weight loss of 5.5% before 150° C. The DVS curve showed that the sample is hygroscopic with a water uptake of 11.5% at 25° C. and 80% RH. A second XRPD pattern performed after DVS showed SCY-078 mesylate Type A had no form change.

TABLE 13 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 3.460873 6386.923000 0.076752 25.53007 100.00 5.977580 779.662100 0.063960 14.78574 12.21 7.966391 2528.494000 0.089544 11.09839 39.59 9.108506 1390.758000 0.089544 9.70916 21.78 9.950825 1211.786000 0.102336 8.88911 18.97 11.611990 1031.845000 0.076752 7.62094 16.16 11.923410 791.823600 0.102336 7.42258 12.40 12.425240 1268.865000 0.089544 7.12391 19.87 13.944550 688.156900 0.102336 6.35096 10.77 14.380410 848.725700 0.102336 6.15943 13.29 14.769580 2671.218000 0.089544 5.99800 41.82 15.556400 1399.797000 0.102336 5.69636 21.92 15.933420 664.503200 0.102336 5.56241 10.40 16.662350 540.586700 0.102336 5.32067 8.46 17.361270 955.468500 0.127920 5.10802 14.96 18.265030 453.378300 0.153504 4.85726 7.10 18.734480 812.380000 0.102336 4.73660 12.72 19.019690 850.304100 0.102336 4.66621 13.31 19.260210 762.325000 0.153504 4.60847 11.94 19.645310 613.773700 0.127920 4.51900 9.61 20.566280 436.518500 0.127920 4.31867 6.83 21.974920 571.254200 0.127920 4.04491 8.94 22.483420 374.000100 0.255840 3.95457 5.86 24.101950 210.447500 0.153504 3.69255 3.29 25.009440 137.497300 0.204672 3.56058 2.15 26.043990 151.416700 0.204672 3.42144 2.37 27.012060 72.724460 0.307008 3.30098 1.14 28.443410 214.270100 0.153504 3.13804 3.35 34.283540 45.493190 0.511680 2.61568 0.71

Example 20

Preparation and Characterization of Scaled-Up SCY-078 Phosphate Type A: To scale up SCY-078 phosphate Type A, a solution of phosphoric acid (87.2 mg) and SCY-078 MeOH desolvate (501.1 mg) in EtOH/EtOAc/acetic acid/H₂O (6.0 mL, 5:3:0.15:0.1; v/v/v/v) was prepared. The suspension was then stirred (500 rpm) at room temperature for 28 hours. Following stirring, some slurry was filtered and the isolated solid was checked by XRPD and named as SCY-078 phosphate Type A. Finally, the suspension was filtered and dried at 30° C. for 4 hours in vacuum before characterization.

Characterization of scaled-up SCY-078 phosphate Type A included XRPD, DSC, TGA, and DVS (FIGS. 37-39). The XRPD pattern of the scaled-up SCY-078 phosphate Type A was compared with another phosphate sample. The comparison XRPD pattern evidenced certain peak shifts, which are signaled with an asterisk. The 2 theta and d-spacing values of SCY-078 phosphate Type A are summarized in Table 14. The DSC curve showed two endotherms at 43.5° C. and 261.6° C. (onset temperatures). The TGA curve showed a weight loss of 5.7% before 100° C. The DVS curve showed that the sample is hygroscopic with a water uptake of 12.5% at 25° C. and 80% RH. A second XRPD pattern performed after DVS showed SCY-078 phosphate Type A had no form change.

TABLE 14 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 3.510749 3822.958000 0.063960 25.16747 100.00 3.777221 1538.172000 0.089544 23.39256 40.24 6.351771 817.179700 0.089544 13.91551 21.38 6.974826 213.577600 0.153504 12.67379 5.59 7.884021 2248.860000 0.089544 11.21415 58.83 8.064520 922.909900 0.076752 10.96356 24.14 9.551988 811.444000 0.179088 9.25936 21.23 9.893185 731.339200 0.127920 8.94077 19.13 11.410930 713.463600 0.153504 7.75476 18.66 11.807790 1002.811000 0.127920 7.49500 26.23 12.234010 806.819600 0.153504 7.23483 21.10 12.657670 895.228700 0.127920 6.99361 23.42 14.562960 1550.900000 0.102336 6.08263 40.57 15.413370 939.681700 0.204672 5.74889 24.58 15.841220 831.733000 0.307008 5.59457 21.76 17.560630 1113.197000 0.409344 5.05048 29.12 18.775820 475.510700 0.153504 4.72626 12.44 20.576380 320.212100 0.307008 4.31657 8.38 22.143260 373.653900 0.204672 4.01454 9.77

Example 21

Preparation and Characterization of Scaled-Up SCY-078 Citrate Type A (Molar Equivalency—counter ion/API 1.0): To scale up SCY-078 citrate Type A, a solution of citric acid (130.7 mg) and SCY-078 MeOH desolvate (501.6 mg) in ACN (15.0 mL) was prepared. The suspension was then stirred (500 rpm) at room temperature for 30 hours. Following stirring, some slurry was filtered and the isolated solid was checked by XRPD to confirm SCY-078 citrate Type A. Finally, the suspension was filtered and dried at 30° C. for 4 hours in vacuum before characterization.

Characterization of scaled-up SCY-078 citrate Type A included XRPD, DSC, TGA, and DVS (FIGS. 40-42). XRPD pattern evidenced that SCY-078 citrate Type A was successfully scaled up. The 2 theta and d-spacing values are summarized in Table 15. The DSC curve showed an endotherm at 38.5° C. and a melting endotherm at 183.7° C. (onset temperatures). The TGA curve showed a weight loss of 4.1% before 110° C. The DVS curve showed that the sample is hygroscopic with a water uptake of 6.4% at 80% RH, 25° C. A second XRPD pattern performed after DVS showed SCY-078 citrate Type A had no form change.

TABLE 15 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 5.448098 1112.016000 0.102336 16.22147 25.78 7.430264 4314.252000 0.153504 11.89794 100.00 8.687142 329.877800 0.153504 10.17913 7.65 9.219851 511.870400 0.153504 9.59216 11.86 9.657531 424.140300 0.255840 9.15840 9.83 10.883410 1078.439000 0.051168 8.12942 25.00 11.515250 2094.987000 0.115128 7.68474 48.56 13.187380 2396.469000 0.089544 6.71386 55.55 14.126680 799.280200 0.255840 6.26949 18.53 14.845300 757.431100 0.230256 5.96758 17.56 16.818410 2913.176000 0.127920 5.27165 67.52 18.364160 492.518400 0.153504 4.83127 11.42 19.381840 490.181800 0.307008 4.57983 11.36 20.774420 185.062500 0.307008 4.27587 4.29 22.213250 308.712600 0.204672 4.00205 7.16 23.587350 109.338400 0.818688 3.77193 2.53 29.871450 85.095400 0.307008 2.99120 1.97

Example 22

Chemical Characterization of SCY-078 Salts: The chemical purity of each of the scaled-up salts of SCY-078 (hippurate Type B, fumarate Type A, mesylate Type A, phosphate Type A, and citrate Type A) was tested using HPLC chromatographs. The chromatographs of the five compounds indicated that each compound has purity greater than 99% (Table 16).

TABLE 16 Chemical Characterization of SCY-078 Salts Compound Purity Hippurate Type B 99.19 Fumarate Type A 99.90 Mesylate Type A 99.91 Phosphate Type A 99.91 Citrate Type A 99.89

Example 23

Evaluation of the pH Value of SCY-078 Salts in Water: The pH value for the saturated salt solutions of SCY-078 (i.e., hippurate Type B, fumarate Type A, mesylate Type A, phosphate Type A, and citrate Type A) was tested. To test the pH, a solution of each compound was equilibrated at room temperature using a rolling incubator (25 rpm) for 1 hour and 24 hours before measurement. The results (Table 17) suggested that the pH values of each of the salts tested is in the range of 3.0 to 5.0.

TABLE 17 pH value of saturated SCY-078 salt solutions in H₂O Salt Form pH 1 hr pH 24 hrs Hippu rate Type B 4.6 4.2 Fumarate Type A 4.4 3.8 Mesylate Type A 3.4 3.6 Phosphate Type A 3.4 3.6 Citrate Type A 3.5 3.8

Example 24

Evaluation of the Kinetic Solubility of SCY-078 Salts: The kinetic solubilities of SCY-078 hippurate Type B, SCY-078 fumarate Type A, SCY-078 mesylate Type A, SCY-078 phosphate Type A, and SCY-078 citrate Type A were measured in dextrose buffer at pH 5.5, phosphate buffer at pH 6.0, phosphate buffer at pH 7.5, SGF media, FeSSIF media, and FaSSIF media according to the method described above. After filtration, 0.2 mL of supernatant was collected for HPLC quantification. The remaining solution was collected for pH measurement. The remaining solid was collected for XRPD characterization. The results appear in Table 18.

TABLE 18 Kinetic Solubility (mg/mL) of SCY-078 salts Hippu- Fuma- Mesy- Phos- Cit- Time rate rate late phate rate Media (hours) Type B Type A Type A Type A Type A dextrose 1 hr 0.54 0.05 1.4 0.10 0.67 buffer 4 hrs 1.7 0.46 2.5 0.09 4.2 (pH 5.5) 24 hrs 4.4 2.1 4.3 0.04 8.3 phosphate 1 hr <LOQ 0.07 0.16 <LOQ 0.75 buffer 4 hrs 0.04 1.2 0.13 <LOQ 4.7 (pH 6.0) 24 hrs 0.47 4.7 0.04 <LOQ 7.5 phosphate 1 hr <LOQ <LOQ <LOQ <LOQ <LOQ buffer 4 hrs <LOQ <LOQ <LOQ <LOQ <LOQ (pH 7.5) 24 hrs <LOQ <LOQ <LOQ <LOQ <LOQ SGF 1 hr 12.4 13.4 17.8 9.2 20.6 4 hrs 15.0 17.7 20.5 16.0 21.4 24 hrs 21.8 23.4 22.5 21.2 21.1 FeSSIF 1 hr 3.4 2.2 1.2 0.89 0.39 4 hrs <LOQ 0.51 1.5 1.3 2.5 24 hrs 0.003 0.02 1.6 1.5 4.2 FaSSIF 1 hr <LOQ <LOD <LOQ 0.006 9.5 4 hrs 0.0007 2.8 0.29 0.17 20.7 24 hrs <LOQ 17.0 0.44 1.0 21.6 LOD: 0.07 μg/mL LOQ: 0.22 μg/mL

Example 25

Evaluation of the Stability of SCY-078 Salts: To test the chemical and physical stability of the salts, samples of the salts were placed under three different conditions for one week: (1) open dish at 25° C. with 60% RH; (2) open dish at 40° C. with 75% RH; and (3) closed dish at 60° C. with no humidity control.

The chemical and physical stability of SCY-078 fumarate Type A and SCY-078 citrate Type A were tested as described above (Table 19). XRPD indicated that neither SCY-078 fumarate Type A nor SCY-078 citrate Type A experienced form change during assessment.

TABLE 19 Stability result of SCY-078 salts Impurity % Sample Condition by HPLC Citrate Initial 0.34 Type A 25° C./60% RH - 1 week 0.09 40° C./75% RH - 1 week 0.10 60° C. - 1 week 0.17 Fumarate Initial 0.12 Type A 25° C./60% RH - 1 week 0.09 40° C./75% RH - 1 week 0.10 60° C. - 1 week 0.10

Example 26

Scaled-up SCY-078 Citrate Type A: A second scale-up of SCY-078 Citrate Type A was carried out to obtain 2.5 g via reactive ACN. To scale-up, SCY-078 MeOH desolvate (2.5 g) and citric acid (660 mg) were dissolved in ACN (80 mL). The resulting solution was stirred at a rate of 1000 rpm at room temperature for 30 hours and then the solid was isolated. The solid obtained was dried at 50° C. under vacuum overnight.

An XRPD pattern (FIG. 43) showed that SCY-078 citrate Type A was successfully scaled up and that it is highly crystalline. The 2 theta and d-spacing values are summarized in Table 20. DSC curve (FIG. 44) exhibited two endothermic peaks at 56.7° C. and 187.1° C. (onset temperatures). TGA curve (FIG. 44) showed 7.9% weight loss before 150° C.

TABLE 20 Pos. Height FWHM Left d-spacing Rel. Int. [°2Th.] [cts] [°2Th.] [Å] [%] 5.400273 434.322700 0.102336 16.36502 3.34 7.453872 13000.820000 0.191880 11.86031 100.00 9.201639 691.948300 0.204672 9.61110 5.32 10.831710 404.555000 0.153504 8.16811 3.11 11.485080 936.115200 0.179088 7.70486 7.20 12.491050 954.805500 0.179088 7.08652 7.34 13.191360 1776.320000 0.204672 6.71184 13.66 15.020350 1342.537000 0.204672 5.89842 10.33 15.664830 532.278900 0.179088 5.65717 4.09 15.955570 613.057500 0.127920 5.55474 4.72 16.751250 951.729000 0.153504 5.29264 7.32 17.978130 170.323300 0.204672 4.93412 1.31 19.591770 472.971000 0.204672 4.53123 3.64 22.213400 146.982900 0.204672 4.00202 1.13 23.845740 34.469910 0.614016 3.73164 0.27 25.160050 117.741100 0.307008 3.53961 0.91 28.761350 129.234400 0.255840 3.10407 0.99 30.356250 332.945100 0.230256 2.94452 2.56 32.317870 87.151140 0.307008 2.77014 0.67 34.725480 74.664570 0.511680 2.58339 0.57

Example 27

Approximate Solubility of SCY-078 Citrate Type A: The approximate solubility of SCY-078 citrate Type A from Example 26 was determined in 19 solvents at room temperature (25±3° C.) according to the procedure described above and is reported in Table 21 below.

TABLE 21 Solvent Solubility (mg/mL) MeOH >42.4 EtOH <1.0 IPA >40.0 Acetic Acid >41.0 ACN <1.1 Acetone 1.6 < S < 1.7 MIBK <1.0 EtOAc <1.0 IPAc <1.1 MTBE <1.0 THF >41.0 2-MeTHF >41.8 1,4-Dioxane >41.0 NMP >40.6 DMSO >40.6 DCM <1.0 Toluene <1.0 Heptane <1.1 DMAc >40.8

Example 28

Kinetic Solubility of SCY-078 Citrate Type A in Water: The kinetic solubility of SCY-078 citrate Type A from Example 26 was evaluated according to the procedure described above. After the samples were centrifuged, the residual solids analyzed by XRPD and the supernatant concentration measured by HPLC. Results (Table 22) indicated that SCY-078 citrate Type A partially converted to amorphous in water after 24 hours, and exhibited a slow rate of dissolution and increasing solubility in water from 1 hour to 24 hours.

TABLE 22 Kinetic Solubility of SCY-078 citrate Type A in Water Initial 1 hr 4 hrs 24 hrs pH 8.0  3.3  3.3  3.4 Solubility (mg/mL) 18.6 26.7 41.4 Form Citrate Citrate Citrate Citrate Type A + Type A Type A Type A amorphous

Then the kinetic solubility of SCY-078 citrate Type A was compared with a mixture of SCY-078 amorphous freebase/citric acid. The kinetic solubility comparison was conducted in water at room temperature and was measured at 1 hour, 4 hours, and 24 hours with a ratio of solute to solvent of 20 mg/mL and 50 mg/mL. Results (Table 23) indicated that SCY-078 citrate Type A shows higher dissolution rate and equilibrium solubility in water than the mixture of SCY-078 amorphous freebase/citric acid.

TABLE 23 Kinetic solubility comparison between mixture of SCY-078 freebase/citric acid and SCY-078 citrate Type A Solubility Starting Material Time pH XRPD Form (mg/ML) Freebase Citric Acid 1 hr 2.7 Amorphous 3.0 20 mg/mL 4 hrs 2.9 Amorphous 5.1 24 hrs 3.5 Amorphous 10.1 Freebase Citric Acid 1 hr 2.7 Amorphous 2.9 50 mg/mL 4 hrs 2.8 Amorphous 16.8 24 hrs 3.2 Amorphous 28.1 Citrate Type A 20 mg/mL 1 hr 3.5 Citrate Type A 3.5 4 hrs 3.4 Citrate Type A 8.3 24 hrs 3.3 Citrate Type A 17.0 Citrate Type A 50 mg/mL 1 hr 3.4 Citrate Type A 6.5 4 hrs 3.3 Citrate Type A 14.5 24 hrs 3.2 Citrate Type A 33.9

The remaining solids from the solubility measurement of physical mixture of freebase:citric acid at 1:1 molar ratio in water were amorphous. To determine the form of the amorphous, liquid NMR was performed on a Bruker 400M NMR Spectrometer using CD₃OD. The spectrum showed the number of hydrogen atoms assigned to citric acid as 3.12, corresponding to 0.78 equivalent of citric acid. This is less than 1:1 for mono-citrate and suggests it is a mixture that comprises a majority of amorphous citrate salt with a small quantity of amorphous freebase.

Example 29

Equilibrium Solubility of SCY-078 Salts in Water: Equilibrium solubility of SCY-078 citrate Type A from Example 26 in water was determined using the method described above. Results showed that SCY-078 citrate Type A exhibits 38.1 mg/mL solubility in water with the undissolved material having become amorphous after stirring in water for 24 hours.

Then to further study the solubility of SCY-078 citrate Type A in water, citrate Type A was tested for 24 hours at three ratios of solute to solvent: 0.3 mg/mL, 2.0 mg/mL, and 50.6 mg/mL. Each test used magnetic stirring and began with an initial pH of 8.0. The results (Table 24) indicated that SCY-078 citrate Type A exhibits a concentration-dependent solubility in water.

TABLE 24 Concentration dependent solubility of SCY-078 citrate Type A in water Ratio of Solid/Solvent Solubility after 24 hrs (mg/mL) (mg/mL) Final pH 0.3 <0.3 5.1 (suspension) 2.0 >2.0 4.2 (clear) 50.6 38.1 3.4

Example 30

Kinetic Solubility of SCY-078 Citrate Amorphous in Water: SCY-078 citrate amorphous was prepared under various conditions, including lyophilization from phosphate buffer (pH 6.0), lyophilization from water, and fast evaporation in THF. The results appear in Table 25. Where the table indicates “limited solid,” there was not sufficient solid for XRPD analysis of the solid form.

Lyophilization in phosphate buffer (pH 6.0)—For lyophilization in phosphate buffer (pH 6.0), SCY-078 citrate amorphous was prepared by first preparing 50 mM pH 6.0 buffer. Then citrate Type A (30 mg) was weighed into a 20-mL vial. Then pH 6.0 buffer (20 mL) was added to the vial and was stirred at room temperature for 24 hours. The samples were filtered and the supernatant was cooled to −15° C. for 2 hours. Finally, the frozen samples were lyophilized at −50° C. for 12 hours. A larger batch (150 mg) was prepared using the same procedure noted above. The kinetic solubility of the second batch of the lyophilized product in water was measured according to the above procedure except that ˜150 mg of solid and 1.0 mL of water were used. After the samples were centrifuged, the residual solids were analyzed by XRPD and the supernatant concentration was measured by HPLC.

Lyophilization in water—For lyophilization in water, amorphous citrate was prepared by first weighing citrate Type A (˜150 mg) into a 20-mL vial. Then 10 mL of water was added to dissolve the solid completely. The solution was filtered and put in a condition of −20° C. until it froze. Finally, the sample was lyophilized under -50° C. for 24 hours. The kinetic solubility of the lyophilized product in water was measured according to the above procedure except that ˜120 mg of solid and 1.5 mL of water were used. After the samples were centrifuged, the residual solids were analyzed by XRPD and the supernatant concentration was measured by HPLC.

Fast Evaporation in THF—For fast evaporation in THF, amorphous citrate was prepared by first weighing citrate Type A from Example 26 (˜150 mg) into a 20-mL vial. Then THF (3 mL) was added to dissolve the solid completely. The solution was filtered under a fume hood for performance of fast evaporation. The kinetic solubility in water of the product in water was measured according to the above procedure except that ˜120 mg of solid and 1.5 mL of water were used. After the samples were centrifuged, the residual solids were analyzed by XRPD and the supernatant concentration was measured by HPLC.

TABLE 25 Kinetic solubility of amorphous SCY-078 citrate in water Solubility Starting Solid Time (mg/mL) pH Form Lyophilization in pH 6.0 buffer  1 hr 0.14 5.1 Amorphous  4 hrs 0.39 5.1 Amorphous 24 hrs 1.5 5.1 Amorphous Lyophilization in water (pH 8.0)  1 hr 55.4 3.1 Amorphous  4 hrs 54.7 3.3 NA 24 hrs 55.5 3.2 NA Fast evaporation in THF  1 hr 44.5 3.2 Amorphous (pH 8.0)  4 hrs 50.2 3.2 NA 24 hrs 52.8 3.2 NA NA: Limited solid for XRPD

Example 31

Slow Evaporation of SCY-078 Citrate Type A: Slow evaporation experiments were performed in 12 different solvent systems. SCY-078 citrate Type A from Example 26 (10 mg) was dissolved with solvent (0.05 mL −0.25 mL) for each sample in a 3-mL glass vial. The visually clear solutions were subjected to slow evaporation at room temperature to dryness. The solids obtained were then isolated for XRPD analysis, which showed that no crystalline form was obtained. The results appear in Table 26.

TABLE 26 Slow evaporation experiments Solvent Form MeOH Amorphous IPA Amorphous Acetic Acid Amorphous THF Amorphous MeOH/ACN, 9/1 Amorphous IPA/Acetone, 9/1 Amorphous THF/EtOAc, 9/1 Amorphous MeOH/H₂O, 9/1 Amorphous THF/Acetone, 9/1 Amorphous IPA/DCM, 9/1 Amorphous MeOH/MTBE, 9/1 Amorphous THF/EtOH, 9/1 Amorphous

Example 32

Slurry Conversion of SCY-078 Citrate Type A: Slurry conversion experiments were conducted under 50 conditions. SCY-078 citrate Type A from Example 26 (˜10 mg) was suspended in each solvent (0.5 mL). The suspensions were stirred for 3 days at either room temperature (Table 27) or 50° C. (Table 28). After stirring, the solids were isolated for XRPD analysis. If the suspensions turned into clear solutions upon slurry, the clear solutions were subjected to slow evaporation at room temperature. The results revealed that SCY-078 citrate Type B and mixtures of SCY-078 citrate Type A and SCY-078 citrate Type B were discovered. All SCY-078 citrate Type C that was discovered was identified as freebase form.

TABLE 27 Slurry conversion experiments at RT Solvent Form EtOH Type B ACN Type B Acetone Type B MIBK Type B EtOAc Type B IPAc Type B MTBE Amorphous 1,4-Dioxane Amorphous via slow evaporation DCM Type B Toluene Type B Heptane Type B MeOH/Acetone, 1/19 Type B IPA/Heptane, 1/19 Type B THF/Toluene, 1/19 Type B NMP/EtOH, 1/19 Amorphous NMP/Acetone, 1/19 Amorphous NMP/IPAc, 1/19 Amorphous NMP/DCM, 1/19 Type C DMSO/ACN, 1/19 Type C DMSO/MIBK, 1/19 Type C DMSO/EtOAc, 1/19 Type C DMSO/Toluene, 1/19 Type C DMAc/EtOH, 1/19 Type C DMAc/Acetone, 1/19 Type C DMAc/MTBE, 1/19 Type C

TABLE 28 Slurry conversion experiments at 50° C. Solvent Form EtOH Type B ACN Type B Acetone Type A + B MIBK Type B EtOAc Amorphous IPAc Type B MTBE Amorphous 1,4-Dioxane Amorphous via slow evaporation DCM Type B Toluene Type A + B Heptane Type B MeOH/Acetone, 1/19 Type A + B IPA/Heptane, 1/19 Type B THF/Toluene, 1/19 Type A + B NMP/EtOH, 1/19 Amorphous NMP/Acetone, 1/19 Amorphous NMP/IPAc, 1/19 Amorphous NMP/DCM, 1/19 Amorphous DMSO/ACN, 1/19 Type C DMSO/MIBK, 1/19 Type C DMSO/EtOAc, 1/19 Type C DMSO/Toluene, 1/19 Type C DMAc/EtOH, 1/19 Amorphous DMAc/Acetone, 1/19 Type C DMAc/MTBE, 1/19 Type C

Example 33

Reverse Anti-solvent addition of SCY-078 citrate Type A: Reverse anti-solvent addition experiments were conducted under 14 conditions. SCY-078 citrate Type A from Example 26 (˜10 mg) was dissolved in each solvent (0.1 mL) to obtain a clear solution. This solution was added drop-wise into a glass vial containing 2.0 mL of each anti-solvent at room temperature. The precipitate was isolated for XRPD analysis. Slow evaporation experiments were conducted for the clear solutions. The results, which appear in Table 29, suggested that SCY-078 citrate Type E and SCY-078 citrate Type F were obtained. SCY-078 citrate Type D and SCY-078 citrate Type J were identified as freebase form.

TABLE 29 Reverse anti-solvent addition experiments at RT Anti- Solvent solvent Observation Form MeOH IPAc Precipitation Type E MeOH DCM Clear Amorphous via slow evaporation IPA EtOH Clear Clear solution after slow evaporation RT for 2 weeks IPA MTBE Precipitation Amorphous IPA Toluene Precipitation Type F THF ACN Precipitation Type D THF MIBK Precipitation Amorphous THF Heptane Precipitation Amorphous NMP ACN Clear Clear solution after slow evaporation at RT for 2 weeks NMP Toluene Clear Clear solution after slow evaporation at RT for 2 weeks DMSO EtOH Clear Type J DMSO DCM Clear Amorphous via slow evaporation DMAc DCM Clear Amorphous via slow evaporation DMAc Toluene Clear Clear solution after slow evaporation at RT for 2 weeks

Example 34

Solid vapor diffusion of SCY-078 Citrate Type A: Solid vapor diffusion experiments were conducted using four solvents at room temperature. SCY-078 citrate Type A from Example 26 (˜10 mg) was placed into a 3-mL glass vial. Then the vial was sealed into a 20-mL glass vial with a solvent (3 mL). The system was kept at room temperature for six days, which was sufficient time for organic vapor to interact with the solids. The solids were characterized by XRPD to identify crystalline forms. The results (Table 30) indicated that SCY-078 citrate Type A and SCY-078 citrate Type B were generated.

TABLE 30 Solid vapor diffusion experiments Solvent Form EtOH Type A EtOAc Type B Acetone Type A DCM Type A

Example 35

Solution vapor diffusion of SCY-078 Citrate Type A: Solution vapor diffusion experiments were conducted under 5 conditions at room temperature. SCY-078 citrate Type A from Example 26 (˜10 mg) was dissolved in a solvent to obtain a clear solution in a 3-mL glass vial. The vial was then sealed into a 20-mL glass vial with a volatile anti-solvent (3 mL). The system was kept at room temperature for six days, which allowed sufficient time for precipitation. As no precipitation was observed, the samples were evaporated slowly to dryness at room temperature. The solids were separated and analyzed by XRPD. The results (Table 31) indicated that no crystalline form was obtained.

TABLE 31 Solution vapor diffusion experiments Anti- Solvent solvent Observation Form Acetic Acid EtOH Clear Amorphous via slow evaporation DMSO Acetone Clear Amorphous via slow evaporation DMAc IPAc Clear Clear solution after slow evaporation at RT for 2 weeks IPA DCM Clear Amorphous via slow evaporation NMP MTBE Clear Clear solution after slow evaporation at RT for 2 weeks

Example 36

Polymer Induced Crystallization of SCY-078 Citrate Type A: Polymer induced crystallization experiments were performed under four conditions. SCY-078 citrate Type A from Example 26 (˜10 mg) was dissolved in a solvent (0.1 mL -0.8 mL) in a 3-mL glass vial. A mixed polymer (-1.0 mg) was added into the visually clear solutions. The “mixed polymer” was a mixture of six polymers (polyvinyl alcohol, polyvinylchloride, polyvinyl pyrrolidone, polyvinyl acetate, hypromellose, and methyl cellulose) at the mass ration of 1.0. All the samples were then evaporated slowly at room temperature to dryness. The solids obtained were isolated for XRPD analysis. The results (Table 32) showed that no crystalline form was observed.

TABLE 32 Polymer induced crystallization experiments Solvent Form MeOH Amorphous THF Amorphous 1,4-Dioxane Amorphous MeOH/EtOH, 9/1 Amorphous MeOH/Acetone, 9/1 Amorphous

Example 37

Slow cooling of SCY-078 Citrate Type A: Slow cooling experiments were conducted under 10 conditions (Table 33). SCY-078 citrate Type A from Example 26 (˜10 mg) was suspended in a solvent (0.1 mL -0.2 mL) at 50° C. Suspensions were filtered at 50° C., and the filtrates were collected and cooled from 50° C. to 5° C. at a rate of 0.1° C./min. All solutions were clear and subjected to slow evaporation at room temperature to induce precipitation. The solids were isolated for XRPD analysis. The results (Table 33) indicated that SCY-078 citrate Type C and SCY-078 citrate Type J were produced and that both SCY-078 citrate Type C and SCY-078 citrate Type J are freebase forms.

TABLE 33 Slow Cooling Experiments Observation Solvent, v/v (5° C.) Form MeOH/Toluene, Clear Amorphous 9/1 IPA/ACN, 9/1 Clear Amorphous IPA/EtOAc, 9/1 Clear Amorphous THF/Toluene, 9/1 Clear Amorphous THF/DCM, 9/1 Clear Amorphous NMP/MTBE, 9/1 Clear Amorphous DMSO/Acetone, Clear Type C 9/1 DMAc/IPAc, 9/1 Clear Type J DMAc/ACN, 9/1 Clear Type J

Example 38

SCY-078 Citrate Type A via Reactive Crystallization: SCY-078 citrate Type A was obtained by reactive crystallization in ACN. The XRPD pattern showed distinctive diffraction peaks (FIG. 45). DSC curve showed two endothermic peaks at 56.7° C. and 187.1° C. (onset temperatures) (FIG. 46). TGA curve displayed a 7.9% weight loss up to 150° C. (FIG. 46). DVS plot showed a water uptake of 7.0% at 80% RH (FIG. 47). There was no form change after DVS analysis.

SCY-078 citrate Type A was also tested with variable temperature XRPD analysis. No form change was observed upon heating SCY-078 citrate Type A to 120° C. and then cooling back to 25° C., indicating that SCY-078 citrate Type A is an anhydrate.

After crystallization process development, SCY-078 citrate Type A exhibited higher crystallinity and less surface moisture adsorption was produced (FIG. 49). DSC curve showed two endothermic peaks at 41.7° C. and 194.8° C. (onset temperatures). TGA curve displayed 2.9% weight loss up to 150° C. DVS analysis showed a water uptake of 6.5% at 25° C. and 80% RH. No form change was observed after DVS analysis. ¹H-NMR spectrum in CD₃OD showed the molar ration of freebase and citric acid is 1:1, indicating that SCY-078 citrate Type A is mono-citrate.

Example 39

SCY-078 Citrate Type B: SCY-078 citrate Type B was obtained by slurry conversion at room temperature in ACN. SCY-078 citrate Type B can also be obtained by slurrying SCY-078 citrate Type A in various organic solvents such as EtOH, ACN, acetone, MIBK, EtOAc, IPAc, DCM, toluene, heptane, MeOH/acetone (1/19, v/v), IPA/heptane (1/19, v/v), and THF/toluene (1/19, v/v). SCY-078 citrate Type B converts to Type A rapidly under vacuum or upon N₂ flow at room or elevated temperature.

The XRPD pattern of SCY-078 citrate Type B showed distinctive diffraction peaks (FIG. 51). DSC curve exhibited three endothermic peaks at 70.8° C., 190.6° C., and 202.9° C. (peak temperatures) (FIG. 52). TGA curve displayed 10.3% weight loss up to 150° C. (FIG. 52).

Example 40

SCY-078 Citrate Type E: SCY-078 citrate Type E was obtained by drying a metastable solvate SCY-078 citrate Type R from MeOH/IPAc. The XRPD pattern shows the crystalline form of the sample (FIG. 53).SCY-078 citrate Type E is not stable at ambient conditions, since it rapidly converts to a new form (SCY-078 citrate Type M) after exposing to air for 2 days (FIG. 54).

Example 41

SCY-078 Citrate Type F: SCY-078 citrate Type F was obtained by reverse anti-solvent addition in IPA/toluene according to the process described in Example 33 and Table 29. The XRPD pattern indicated that SCY-078 citrate Type F is weakly crystalline (FIG. 55). DSC curve exhibited a wide endothermic peak at 37.3° C. (onset temperature)(FIG. 56). TGA curve displayed a weight loss of 11.8% up to 120° C. (FIG. 56).

Example 42

SCY-078 Citrate Type M: SCY-078 citrate Type M was obtained by storing SCY-078 citrate Type E in ambient conditions for 2 days. The XRPD pattern of SCY-078 citrate Type M displayed distinctive diffraction peaks (FIG. 57). DSC curve exhibited two endothermic peaks at 125.8° C. and 193.3° C. (onset temperatures) (FIG. 58). TGA curve displayed a 11.4% weight loss up to 150° C. (FIG. 58). DVS plot showed 11.0% water uptake at 25° C. and 80% RH (FIG. 59). After DVS, SCY-078 citrate Type M converts to partially amorphous.

XRPD analysis was also performed at variable temperatures wherein XRPD patterns were produced at 25° C., then at 150° C., and finally, again at 25° C. (FIG. 60). A shift in diffraction peaks was observed, indicating that Type M is probably a channel hydrate.

Example 43

SCY-078 Citrate Type N: SCY-078 citrate Type N was obtained by slurrying SCY-078 citrate Type B in EtOH at room temperature for two weeks. SCY-078 citrate Type N can also be obtained by exposing SCY-078 citrate Type A in EtOH vapor for 8 days or slurrying SCY-078 citrate Type A in EtOH for 2 hours. The XRPD pattern of SCY-078 citrate Type N indicates that it is highly crystalline (FIG. 61). SCY-078 citrate Type N converts to SCY-078 citrate Type A after vacuum drying at room temperature (FIG. 62), indicating SCY-078 citrate Type N is a metastable EtOH solvate, which rapidly converts to SCY-078 citrate Type A under vacuum or upon air/N₂ drying at ambient temperature or elevated temperature.

Example 44

SCY-078 Citrate Type O: SCY-078 citrate Type O was obtained by slurrying SCY-078 citrate Type M in acetone at room temperature for 19 hours. The XRPD pattern of SCY-078 citrate Type O indicated that it is highly crystalline with distinctive diffraction peaks (FIG. 63). SCY-078 citrate Type O converts to SCY-078 citrate Type S under ambient or vacuum conditions.

Example 45

SCY-078 Citrate Type Q: SCY-078 citrate Type Q was obtained when performing reactive crystallization of freebase and citric acid (1:1) in EtOH without seeds. SCY-078 citrate Type N was consistently obtained when the reactive crystallization was performed using SCY-078 citrate Type N or SCY-078 citrate Type A seeds. The XRPD pattern showed SCY-078 citrate Type Q is highly crystalline with distinctive diffraction peaks (FIG. 64). SCY-078 citrate Type Q can convert to SCY-078 citrate Type A after vacuum drying at room temperature, indicating that SCY-078 citrate Type Q is a metastable EtOH solvate (FIG. 65).

The stability of the two EtOH solvates, SCY-078 citrate Type N and SCY-078 citrate Type Q, was evaluated by measuring their solubility at 5° C. and 20° C. (Table 34). The solubility was measured by slurrying SCY-078 citrate Type N and SCY-078 citrate Type Q samples in EtOH for 24 hours with a magnetic stirring rate of 1000 rpm. SCY-078 citrate Type Q exhibited lower solubility than SCY-078 citrate Type N in EtOH at 5° C. and 20° C., indicating that SCY-078 citrate Type Q is thermodynamically more stable in EtOH from 5° C. to 20° C. XRPD analysis of the remaining wet cakes from the solubility experiments showed no form change for both SCY-078 citrate Type Q and SCY-078 citrate Type N.

TABLE 34 Solubility (mg/mL) of SCY-078 citrate Type N and SCY-078 citrate Type Q in EtOH at different temperatures Temperature (° C.) Type N Type Q 5 24.4 17.4 20 27.1 25.9

Example 46

SCY-078 Citrate Type R: SCY-078 citrate Type R was obtained by slurrying SCY-078 citrate Type M in MeOH/IPAc (1/14, v/v) for 17 hours. SCY-078 citrate Type R can also be obtained by reverse anti-solvent addition in MeOH/IPAc. The XRPD pattern indicated that SCY-078 citrate Type R is weakly crystalline (FIG. 66). XRPD analysis also indicated that SCY-078 citrate Type R is a metastable solvate that can easily convert to SCY-078 citrate Type S upon air drying and to SCY-078 citrate Type M after vacuum drying (FIG. 67).

Example 47

SCY-078 Citrate Type S: SCY-078 citrate Type S can be obtained by drying SCY-078 citrate Type O sample under ambient or vacuum condition. The XRPD patterns showed shift of diffraction peaks after conversion from SCY-078 citrate Type O to SCY-078 citrate Type S (FIG. 68). DSC curve exhibited two endothermic peaks at 35.7° C. and 188.0° C. (onset temperatures) (FIG. 69). TGA curve displayed 6.6% weight loss up to 100° C. (FIG. 69). DVS plot showed 8.2% water uptake at 25° C. and 80% RH for SCY-078 citrate Type S (FIG. 70). XRPD analysis after DVS showed peak shifts. XRPD analysis was further performed at variable temperatures 30° C. to 120° C. and back to 40° C., which showed a shift of diffraction peaks (FIG. 71).

Example 48

Disproportionation of SCY-078 Citrate Salt: Crystalline forms of SCY-078 citrate named Type C, Type I, Type J, and Type P were observed either during polymorph study or when investigating the inter-conversion relationship of different SCY-078 citrate forms. XRPD patterns of the four forms (FIG. 72) demonstrated that SCY-078 citrate Type C and SCY-078 citrate Type J are freebase forms and that SCY-078 citrate Type I and SCY-078 citrate Type P are likely freebase forms.

SCY-078 citrate Type I was obtained in DMSO/EtOH system. SCY-078 citrate Type P was obtained by slurrying SCY-078 citrate Type M in acetone/H₂O system. SCY-078 citrate Type A disproportionated to the freebase (SCY-078 citrate Type C, which converts to SCY-078 citrate Type J upon drying) when slurrying in EtOH/H₂O, acetone/H₂O, and BuOH/H₂O systems. Slurrying SCY-078 citrate Type A or evaporating the citrate solution in DMSO, DMAc and DCM-related co-solvents also resulted in disproportionation.

Example 49

Inter-conversion Between SCY-078 Citrate Type A and Citrate Type B: Slurry experiments were performed with SCY-078 citrate Type A in different organic solvents in order to understand the inter-conversion between SCY-078 citrate Type A and SCY-078 citrate Type B. SCY-078 citrate Type A (-20 mg) was suspended into a solvent (0.5 mL) in a 1.5-mL glass vial. After the suspensions were ultrasonicated for 1 hour or stirred for 6 hours at room temperature, the remaining solids were isolated for XRPD analysis. The results (Table 35) indicated that SCY-078 citrate Type B can be obtained from various organic solvents. SCY-078 citrate Type B was also prepared from SCY-078 citrate Type A by slurry in EtOH, ACN, acetone, MIBK, EtOAc, IPAc, DCM, toluene, heptane, MeOH/acetone (1/19), IPA/heptane (1/19), THF/toluene (1/19) or by solid vapor diffusion in EtOAc. SCY-078 citrate Type B can convert to SCY-078 citrate Type A via drying under N₂ or vacuum at room temperature.

TABLE 35 Summary of preparation methods of SCY-078 citrate Type B Form, Vacuum Form, Wet filtered Form after N₂ Solvent Preparation Slurry slurry drying at RT ACN Ultrasonication for 1 hr Type B Type A Type A DCM Ultrasonication for 1 hr Type B Type A Type A EtOAc Stirring for 6 hrs Type B Type B Type A

Example 50

Inter-conversion between SCY-078 citrate Type A and SCY-078 citrate Type N and SCY-078 citrate Type Q: SCY-078 citrate Type N can be obtained by slurrying SCY-078 citrate Type A (or SCY-078 citrate Type B) in EtOH with ultrasonication or at room temperature for 1 hour. SCY-078 citrate Type N rapidly converts to SCY-078 citrate Type A via vacuum filtration (FIG. 73).

SCY-078 citrate Type Q was obtained by reactive crystallization of freebase MeOH desolvate and citric acid (1:1) in EtOH without seeds. After drying in vacuum at room temperature, SCY-078 citrate Type Q converts to SCY-078 citrate Type A (FIG. 74). The study of the inter-conversion between SCY-078 citrate Type A and SCY-078 citrate Type N and SCY-078 citrate Type Q is summarized below in Table 36.

TABLE 36 Slurry conversion of SCY-078 citrate Type A or SCY-078 citrate Type B to SCY-078 citrate Type N Form, Form, Starting Material Method Solvent Wet Slurry Vacuum Dry Type A Ultrasonication EtOH Type N Type A Type B Ultrasonication EtOH Type N Not Measured MeOH Reactive EtOH Type Q Type A desolvate/citric crystallization acid (1:1)

Example 51

Inter-conversion Relationship Around Channel Hydrate SCY-078 Citrate Type M: Metastable solvate SCY-078 citrate Type R was obtained by reverse anti-solvent addition in MeOH/IPAc. SCY-078 citrate Type R converted to SCY-078 citrate Type E after drying in vacuum at room temperature, and then SCY-078 citrate Type M was obtained by storing SCY-078 citrate Type E under ambient conditions for 2 days (FIG. 75). SCY-078 citrate Type R was found to convert to SCY-078 citrate Type M directly upon vacuum drying at room temperature.

Slurry experiments were performed on SCY-078 citrate Type M in selected organic solvents. The results (Table 37) indicated that SCY-078 citrate Type M converted to SCY-078 citrate Type 0 after slurrying in acetone for 17 hours, and SCY-078 citrate Type 0 converted to SCY-078 citrate Type S after air drying (FIG. 76). SCY-078 citrate Type M converted to SCY-078 citrate Type A when slurried in ACN (FIG. 77), and converted to metastable solvate SCY-078 citrate Type R in MeOH/IPAc co-solvent (FIG. 78). SCY-078 citrate Type R converted to SCY-078 citrate Type S upon air drying and converted back to SCY-078 citrate Type M through vacuum drying at room temperature. No form change was observed by slurrying SCY-078 citrate Type M in heptane (FIG. 79).

TABLE 37 Slurry experiments result of SCY-078 citrate Type M in organic solvents Solvent Condition Solid Form Acetone 10 mins Amorphous ultrasonication/wet 17 hrs (slurry) wet Type O 17 hrs (slurry) dry under Type S ambient conditions ACN 10 mins Type M ultrasonication/wet 17 hrs (slurry) wet Type B 17 hrs (slurry) dry under Type A ambient conditions Heptane 10 mins Type M ultrasonication/wet 17 hrs (slurry) wet Type M 17 hrs (slurry) dry under Type M ambient conditions MeOH/IPAc 10 mins Type M (5/70, v/v) ultrasonication/wet 17 hrs (slurry) wet Type R* 17 hrs (slurry) dry under Type S ambient conditions *Type R converts to Type M after vacuum drying at RT

Example 52

Stability Study of SCY-078 Citrate Type A, SCY-078 Citrate Type M, and SCY-078 Citrate Type S: As described in Example 25 and Table 19, SCY-078 citrate Type A is physically/chemically stable at the tested conditions for at least 1 week. To test the physical and chemical stability of SCY-078 citrate Type M and SCY-078 citrate Type S, each was placed under three different conditions: (1) open dish at 25° C. with 60% RH; (2) open dish at 40° C. with 75% RH; and (3) closed dish at 60° C. with no humidity control. SCY-078 citrate Type M was tested for 4 days and SCY-078 citrate Type S was tested for 1 week (FIGS. 80-81).

The results (Table 38) showed that SCY-078 citrate Type M was physically and chemically stable at 25° C. and 60% RH for at least 4 days. One diffraction peak change and partial crystallinity loss was observed in the XRPD pattern of SCY-078 citrate Type M after storage at 40° C. and 75% RH. This is consistent with the previous observation in Example 42 that SCY-078 citrate Type M partially converts to amorphous after DVS analysis. The impurity of SCY-078 citrate Type M increased under closed conditions at 60° C. for 4 days.

SCY-078 citrate Type S is physically and chemically stable under 60° C. closed conditions for one week. Diffraction peak shifts were observed for the samples stored at 25° C. and 60% RH and 40° C. and 75% RH.

TABLE 38 Physical and Chemical Stability of SCY-078 citrate Type M and SCY-078 citrate Type S Impurity % Solid Sample Condition by HPLC Form Type M Initial 0.42 Type M (4 days) 25° C./60% 0.39 Type M RH 40° C./75% 0.00 Type M* RH 60° C. closed 0.63 Type M Type S Initial 0.10 Type S (1 week) 25° C./60% 0.03 Type S** RH 40° C./75% 0.03 Type S** RH 60° C. closed 0.11 Type S *One peak change was observed for the sample **Peak shifts were observed for the samples

Example 53

Alternative Preparation of SCY-078 Citrate Type A: A 10-L reactor was charged with SCY-078 phosphate (450 g; freebase content in phosphate was 85.6% by HPLC). 2-MeTHF (2.25 L) was charged into the same reactor. A 10% Na₂CO₃ water solution (2.25 L) at 20° C. was charged into the reaction in 25 min. The suspension was stirred at 20° C. for 20 min and then allowed to settle for 30 min. The organic layer was collected and washed with 1.8 L of saturated NaCl water solution twice, and then further washed with 1.8 L deionized water once.

The organic layer was transferred to a 4-L crystallizer. The reactor was rinsed with 250 mL 2-MeTHF and the liquid was transferred into the crystallizer containing the organic layer. The solution was concentrated in the crystallizer to 900 mL at 50° C. The crystallizer was charged with 900 mL methanol and the mixture was cooled to 40° C. The mixture was stirred at 40° C. for 1 hour (clear). 4.5 g of seeds were added to the crystallizer and the suspension was aged at 40° C. for 1 hour. The mixture was then concentrated to 900 mL at 40° C. The crystallizer was then charged with 900 mL methanol and again concentrated to 900 mL at 40° C. This step was repeated twice more and the mother liquor was assayed by gas chromatography. The mixture was cooled to 10° C. in 2 hours and then aged at 10° C. for no less than three hours. The mother liquor was sampled for solution concentration by HPLC. The suspension was filtered and the cake was dried in a vacuum over at 35° C. for 12 hours.

To generate the citrate salt, a 10-L jacketed crystallizer with a twin-impeller over-head agitator was used. The diameter of the impeller is 13 cm. First, EtOH (500 mL) was added into a 10-L crystallizer (Crystallizer 1) and was agitated (300 rpm). The temperature of Crystallizer 1 was maintained at 25° C. The SCY-078 freebase (242.09 g) was added to Crystallizer 1. Another volume of EtOH (500 mL) was charged into Crystallizer 1. Crystallizer 1 was heated to 50° C. A citric acid solution, prepared by dissolving citric acid (58.22 g) into EtOH (758 mL), was charged into Crystallizer 1 in 35 min. Crystallizer 1 was heated to 55° C. and stirred for 20 minutes. Then Crystallizer 1 was cooled to 50° C. for 20 minutes.

After cooling, the extraneous matter was filtered (pore size of 30˜50 μm) and the filtrate was transferred to another 10-L crystallizer (Crystallizer 2). The filter was washed with EtOH (5 mL) and transferred into Crystallizer 2. The mixture in Crystallizer 2 was stirred at 50° C. for 30 minutes. Next a seed slurry, which was prepared from seeds (13.22 g) that were sonicated and dispersed in 50/50 EtOH/n-heptane (68 mL), was rapidly charged into Crystallizer 2. The mixture in Crystallizer 2 was aged at 50° C. for 2 hours. Crystallizer 2 was then charged with n-heptane (1758 mL) for 12 hours at 50° C. The mixture was again aged at 50° C. for 2 hours. From the resulting mixture, a sample was taken for XRPD analysis and microscopy.

The mixture was cooled to 20° C. in 2 hours and then stirred at 20° C. for 3 hours. The batch was filtered and the cake was washed with a solution of 1:1 EtOH/n-heptune (500 mL). The cake was blown with N₂ for 60 minutes. Finally, the cake was dried at 45-55° C. with N₂ blowing.

Ultimately 241 grams of product was obtained with a 86.4% yield. XRPD analysis showed that the product was highly crystalline SCY-078 citrate Type A (FIG. 82). TGA curve showed a weight loss of 2.2% before 150° C. (FIG. 83). The DSC curve showed a melting point of 197.8° C. (onset temperature) (FIG. 83). The resulting crystals were rod-like with an average size of 34.2 pm (FIG. 84).

Example 54

Preparation and characterization of SCY-078 Trifluoroacetate Type A: SCY-078 amorphous freebase (994.3 mg) and trifluoroacetic acid (freebase/acid molar ratio—1/1) were weighted into a 5-mL vial, followed by addition of 5 mL acetonitrile. The mixture was slurried at RT with a magnetic stirring rate of 1000 rpm for 4 days. The suspension was centrifuged and vacuum dried at RT overnight.

SCY-078 Trifluoroacetate Type A is highly crystalline as shown in the XRPD (FIG. 85). A weight loss of 1.1% is observed up to 120° C. in the TGA curve (FIG. 86). The DSC (FIG. 86) shows two endothermic peaks, one at 65.8° C. and 229.8° C. Trifluoroacetate Type A converted to Type B after stored under ambient conditions for 2 days (FIG. 87). The molar ratio of trifluoroacetate Type A (acid: freebase) was determined to be 1:1 via HPLC-IC confirmation.

Example 55

Preparation and characterization of SCY-078 Trifluoroacetate Type B: Trifluoroacetic acid (331.5 mg) was added into acetonitrile (8 mL) in a 20-mL glass vial, followed by addition of amorphous SCY-078 freebase Type A (freebase/acid molar ratio =1/1). The mixture was stirred at RT with a magnetic stirring rate of 600 rpm for 24 hours. The suspension was vacuum filtered and dried at RT for 20 hours. Trifluoroacetate Type A was obtained (2.18 g), which converted to Trifluoroacetate Type B after storage at ambient conditions for almost 1 month.

SCY-078 Trifluoroacetate Type B is highly crystalline as shown in the XRPD (FIG. 88). A weight loss of 4.7% is observed up to 120° C. in the TGA curve (FIG. 89). The DSC (FIG. 89) shows two endothermic peaks, one at 92.8° C. and 230.0° C. Due to the reversible conversation of Trifluoroacetate Type A and Type B, the molar ratio of Trifluoroacetate Type B (acid:freebase) is postulated to be 1:1, same as Type A.

From the DVS (FIG. 90), 3.4 wt % of water uptake was observed at 25° C. 80% RH, indicating that Type B is moderately hygroscopic. The DVS revealed potential form change with respect to RH, estimated to be between 30% RH and 40% RH. SCY-078 Trifluoroacetate Type B converted to Type A after DVS as shown in FIG. 91.

To investigate transition relationship of trifluoroacetate Type A and Type B, both samples were stored in chambers with varying relative humidity to monitor any form change. Summary of trifluoroacetate Type B stored at varying relative humidity is listed in Table 39, and XRPD patterns overlay is displayed in FIG. 92. Trifluoroacetate Type B converted to Type A only at high relative humidity (97% RH) while Type B is stable at low relative humidity (<22% RH). Varying temperature (VT)-XRPD of trifluoroacetate Type A was performed. VT-XRPD patterns overlay is displayed in FIG. 93. Type A converted to Type B after heated to 120° C.

TABLE 39 Relativity Humidity 0% 22% 97% TFA Type B Type B Type B Type A The XRPD patterns were collected after storage for 24 hours.

Example 56

Preparation and characterization of SCY-078 HCl Type I: 342.7 μL of concentrated HCl (37.5%) was dispersed in 40 mL of acetone. 2.0 mg of SCY-078 freebase Type A (freebase/acid molar ratio=1/1.5) was added. The suspension was settled in a biochemical incubator to perform heat-cooling cycles (50° C.˜20° C.) with a magnetic stirring rate of 600 rpm. The suspension was cooled to 5° C. at a rate of 0.1° C./min and aged at 5° C. for 17 hours. The wet cake was vacuum filtered and dried at RT for 20 hours. SCY-078 HCl Type I (2.06 g) was obtained.

SCY-078 HCl Type I is highly crystalline as shown in the XRPD (FIG. 94). A weight loss of 4.2% is observed up to 120° C. in the TGA curve (FIG. 95). The DSC (FIG. 95) shows three endothermic peaks, one at 46.2° C., one at 115.5° C. and one at 274.3° C. The molar ratio of SCY-078 HCl Type I (acid:freebase) was determined to be 1.5:1 via HPLC-IC.

From the DVS (FIG. 96), 6.1 wt % of water uptake was observed at 25° C./80% RH, indicating HCl Type I is moderately hygroscopic. No form change was observed after DVS characterization as shown in FIG. 97.

Example 57

Preparation and characterization of SCY-078 HCl Type II: SCY-078 HCl Type II was obtained by suspending HCl Type I in acetate buffer (pH 5.5) for 4 hours. The XRPD pattern (FIG. 98) indicates Type II is highly crystalline. The TGA shows that HCl Type II exhibits a weigh loss of 6.9% up to 150° C. and the DSC shows an endothermic peak at 48.3° C. (onset temperature), as shown in FIG. 99.

Example 58

The solubility of trifluoroacetate Type A, Type B and HCl Type I was measured in SGF at ambient temperature. Approximately 90 mg of solid sample was weighted into a 4-mL centrifuge tube, and 3 mL of SGF buffer was added before leaving the suspension on a rolling incubator (25 r/min). 1.0 mL aliquot of the suspension was sampled for centrifugation (10000 rpm, 3 mins) the supernatant was analyzed by HPLC and pH measurement and solid by XRPD characterization at 1 hr, 4 hr and 24 hrs, respectively. The results are summarized in Table 40 and the solubility curves are displayed in FIG. 100. All three salts exhibit high solubility in SGF (>20 mg/mL at 24 hrs). Trifluoroacetate Type B converted to Type A in SGF after an hour. However, no form change was observed of trifluoroacetate Type A and HCl Type I in SGF. The XRPD patterns of residual solid are shown in FIG. 101, FIG. 102, and FIG. 103.

TABLE 40 1 hr 4 hrs 24 hrs Starting Form Form Form Material S pH change S pH change S pH change TFA 15.9 2.2 No 19.8 2.2 No 23.2 2.1 No Type A TFA 14.3 2.0 Yes 18.4 2.0 Yes 21.4 1.8 Yes Type B (Type A) (Type A) (Type A) HCl 11.7 1.7 No 17.7 1.9 No 25.1 1.8 No Type I S: solubility, mg/mL.

Example 59

The solubility of trifluoroacetate Type A, Type B and hydrochloride Type I was measured in the FaSSIF alternative media at ambient temperature. Approximately 15 mg of solid sample was weighted into a 4-mL plastic tube, and 3 mL of the media was added before leaving the suspension on a rolling incubator (25 r/min). 1.0 mL aliquot of the suspension was sampled for centrifugation with the supernatant submitted for HPLC and pH measurement and solid for XRPD characterization at 1 hr, 4 hr and 24 hrs, respectively. The results are summarized in Table 41 and the solubility curves are displayed in FIG. 104. All three salts exhibit poor solubility in the FaSSIF alternative media (<0.01 mg/mL at 24the kinetic hrs). Trifluoroacetate Type B converted to Type A after an hour. However, no form change was observed of trifluoroacetate Type A and HCl Type I. The XRPD patterns of residual solid are shown in FIG. 105, FIG. 106, and FIG. 107.

TABLE 41 1 hr 4 hrs 24 hrs Starting Form Form Form Material S pH change S pH change S pH change TFA <LOD 6.5 No <LOD 6.4 No <LOD 6.4 No Type A TFA <LOD 6.4 Yes <LOD 6.4 Yes <LOD 6.3 Yes Type B (Type A) (Type A) (Type A) HCl 0.0033 5.0 No 0.0017 5.3 No 0.0046 5.1 No Type I S: solubility, mg/mL; LOD: 0.00064 mg/mL.

Example 60

The solubility of trifluoroacetate Type A, Type B and HCl Type I was measured in the FeSSIF alternative media at ambient temperature. Approximately 36 mg of solid sample was weighted into a 4-mL plastic tube, and 3 mL of the media was added before leaving the suspension on a rolling incubator (25 r/min). 1.0 mL aliquot of the suspension was sampled for centrifugation with the supernatant submitted for HPLC and pH measurement and solid for XRPD characterization at 1 hr, 4 hr and 24 hrs, respectively. The results are summarized in Table 42 and the solubility curves are displayed in FIG. 108. All three salts exhibit a solubility of ˜3 mg/mL at first one hour. HCl Type I exhibits an equilibrium solubility of 3.5 mg/mL at 24 hrs, while trifluoroacetate (both Type A and Type B) exhibit a decreasing solubility after an hour. Trifluoroacetate Type A converted to Type B after an hour. However, no form change was observed of trifluoroacetate Type B and hydrochloride Type I. The XRPD patterns of residual solid were included in FIG. 109, FIG. 110, and FIG. 111.

TABLE 42 1 hr 4 hrs 24 hrs Starting Form Form Form Material S pH change S pH change S pH change TFA 3.0 5.0 Yes 2.1 5.0 Yes 0.5 5.0 Yes Type A (Type B) (Type B) (Type B) TFA 3.3 5.0 No 3.0 5.0 No 1.1 4.9 No Type B HCl 2.9 4.8 No 3.5 4.8 No 3.5 4.9 No Type I S: solubility, mg/mL.

Example 61

The solubility of trifluoroacetate Type A, Type B and HCl Type I was measured in acetate buffer (pH 5.5) at ambient temperature. Approximately 15 mg of solid sample was weighted into a 4-mL plastic tube, and 3 mL of acetate pH 5.5 buffer was added before leaving the suspension on a rolling incubator (25 r/min). 1.0 mL aliquot of the suspension was sampled for centrifugation with the supernatant submitted for HPLC and pH measurement and solid for XRPD characterization at 4 hr and 24 hrs, respectively. The results are summarized in Table 43 and the solubility curves are displayed in FIG. 112. Trifluoroacetate Type A exhibits higher solubility in acetate pH 5.5 buffer, and no form change was observed. However, Type B converted to Type A in acetate pH 5.5 buffer. While HCl Type I exhibits lower solubility in acetate pH 5.5 buffer comparing with trifluoroacetate, and HCl Type I converted to Type II in acetate pH 5.5 buffer. The XRPD patterns of residual solid were included in FIG. 113, FIG. 114, and FIG. 115.

TABLE 43 4 hrs 24 hrs Starting Form Form Material S pH change S pH change TFA 1.9 5.5 No 3.5 5.4 No Type A TFA 0.89 5.4 Yes 2.2 5.2 Yes Type B (Type A + B) (Type A) HCl 0.59 4.8 Yes 1.1 4.9 Yes Type I (Type II) (Type II) S: solubility, mg/mL.

Example 62

The solubility of trifluoroacetate Type A, Type B and HCl Type I was measured in the phosphate (pH 6.0) alternative media at ambient temperature. Approximately 15 mg of solid sample was weighted into a 4-mL plastic tube, and 3 mL of media was added before leaving the suspension on a rolling incubator (25 r/min). 1.0 mL aliquot of the suspension was sampled for centrifugation with the supernatant for HPLC and pH measurement and solid for XRPD characterization at 4 hr and 24 hrs, respectively. The results are summarized in Table 44, and the solubility curves are displayed in FIG. 116. HCl Type I exhibits higher solubility, while trifluoroacetate Type A exhibits lower solubility. Trifluoroacetate Type A firstly converted to Type B at 4 hrs and back to Type A at 24 hrs, while Type B converted to Type A at 24 hrs. No form change was observed of HCl Type I. The XRPD patterns of residual solid were included in FIG. 117, FIG. 118, and FIG. 119.

TABLE 44 4 hrs 24 hrs Starting Form Form Material S pH change S pH change TFA 0.28 5.8 Yes 0.99 5.5 Back to Form A Type A (Type B) TFA 0.39 5.3 No 2.7 4.9 Yes Type B (Type A) HCl 1.2 3.9 No 4.1 4.4 No Type I S: solubility, mg/mL.

Example 63

Physical and chemical stability evaluation of trifluoroacetate Type A, trifluoroacetate Type B, and HCl Type I was performed at 25° C./60%RH, 40° C./75%RH and 60° C. for 1, 2, 4 and 8 weeks. In the experiments, approximately 20 mg of solid was placed into a 1.5-mL glass vial. The vials were stored under 25° C./60%RH (uncapped), 40° C./75%RH (uncapped) and 60° C. (capped) conditions for 8 weeks. XRPD analysis was then employed to check the crystalline form of the solid, and HPLC was utilized to determine the purity profile at 1, 2, 4 and 8 weeks. From the stability results summarized in Table 45, both trifluoroacetate Type A and HCl Type I are physically and chemically stable under 25° C./60%RH, 40° C./75%RH and 60° C. conditions for 8 weeks. The solid form change of trifluoroacetate Type A at 2 and 4 weeks was postulated to the air-exposure when the sample was taken out for XRPD characterization. Trifluoroacetate Type B is chemically stable, however, physically unstable evidenced by conversion to Type A under these conditions. XRPD patterns overlay of trifluoroacetate Types A and B and HCl Type I at stressed conditions are displayed from FIG. 120 to FIG. 128.

TABLE 45 Initial Time 25° C./60% RH 40° C./75% RH 60° C. (capped) HPLC point HPLC Solid HPLC Solid HPLC Solid Salt purity (weeks) purity form purity form purity form TFA 99.9% 1 99.9% Type A 99.9% Type A 99.9% Type A Type A 2 99.9% Type B 99.9% Type B 99.9% Type B 4 99.9% Type 99.9% Type 99.9% Type A + B A + B A + B 6 — Type A — Type A — Type A 8 99.9% Type A 99.9% Type A 99.9% Type A TFA 99.9% 1 99.9% Type A 99.9% Type A 99.9% Type A Type B 2 99.9% Type A 99.9% Type A 99.9% Type A 4 99.9% Type A 99.9% Type A 99.9% Type A 8 99.9% Type A 99.9% Type A 99.9% Type A HCl 99.9% 1 99.9% Type I 99.9% Type I 99.9% Type I Type I 2 99.9% Type I 99.9% Type I 99.9% Type I 4 99.9% Type I 99.9% Type I 99.9% Type I 8 99.9% Type I 99.9% Type I 99.9% Type I The solid form change of TFA Type A at 2 and 4 weeks was postulated to the air-exposure taken out for XRPD characterization. 

1. A pharmaceutically acceptable salt of compound 1:

wherein the salt is selected from citrate, hippurate, mesylate, and fumarate.
 2. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a chemical purity of at least 99%.
 3. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 2 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5.
 4. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 4 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5.
 5. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 2 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 6. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 4 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 7. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 8 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 8. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 1 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0.
 9. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 4 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0.
 10. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 4 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.
 11. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 7 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.
 12. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 12 mg/mL to 21 mg/mL at 1 hour in SGF media.
 13. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 13 mg/mL to 21 mg/mL at 1 hour in SGF media.
 14. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 18 mg/mL to 21 mg/mL at 1 hour in SGF media.
 15. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 20 mg/mL to 21 mg/mL at 1 hour in SGF media.
 16. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 17 mg/mL to 22 mg/mL at 24 hours in FaSSIF media.
 17. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a kinetic solubility of from 21 mg/mL to 22 mg/mL at 24 hours in FaSSIF media.
 18. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a water sorption of from 2% to 7% at 25° C. and 80% relative humidity as determined by DVS.
 19. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a water sorption of from 3% to 7% at 25° C. and 80% relative humidity as determined by DVS.
 20. The salt of claim 1, wherein the pharmaceutically acceptable salt of compound 1 has a water sorption of from 6% to 7% at 25° C. and 80% relative humidity as determined by DVS.
 21. A citrate salt of compound 1:


22. The citrate salt of claim 21, wherein the citrate salt of compound 1 comprises at least one of Type A, Type B, Type E, Type F, Type M, Type N, Type O, Type Q, Type R, and Type S.
 23. The citrate salt of claim 21, wherein the citrate salt of compound 1 comprises Type A.
 24. The citrate salt of claim 21, wherein the citrate salt of compound 1 consists essentially of Type A.
 25. The citrate salt of claim 21, wherein the citrate salt of compound 1 comprises at least 98% Type A.
 26. The citrate salt of claim 21, wherein the citrate salt of compound 1 comprises at least 99% Type A.
 27. The citrate salt of claim 23, wherein the Type A has an XRPD pattern comprising one or more peaks at d-spacings of 11.86, 7.70, 7.09, 6.71, 5.90, and 5.29 Angstroms.
 28. The citrate salt of claim 21, wherein the Type A has an XRPD pattern comprising one or more peaks at degrees 2 theta of 7.45, 11.49, 12.49, 13.19, 15.02, and 16.75.
 29. The citrate salt of claim 23, wherein the Type A is stable for at least 1 week when stored at 60° C.
 30. The citrate salt of claim 23, wherein the Type A is stable for at least 1 week when stored at 25° C. and 60% relative humidity.
 31. The citrate salt of claim 23, wherein the Type A is stable for at least 1 week when stored at 40° C. and 75% relative humidity.
 32. The citrate salt of claim 23, wherein the Type A has an equilibrium solubility of 38 mg/mL in non-buffered water at room temperature.
 33. The citrate salt of claim 23, wherein the Type A has an approximate solubility of from 40 mg/mL to 42 mg/mL at room temperature in at least one solvent selected from methanol, isopropyl alcohol, acetic acid, tetrahydrofuran, 2-methyl-tetrahydrofuran, 1,4-dioxane, n-methyl-2-pyrrolidone, dimethyl sulfoxide, and dimethylacetamide.
 34. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 4 mg/mL at 4 hours in dextrose buffer at pH 5.5.
 35. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 8 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 36. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 5 mg/mL at 4 hours in phosphate buffer at pH 6.0.
 37. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.
 38. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 21 mg/mL at 1 hour in SGF media.
 39. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 4 mg/mL at 24 hours in FeSSIF media.
 40. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 10 mg/mL at 1 hour in FaSSIF media.
 41. The citrate salt of claim 23, wherein the Type A has a kinetic solubility of 21 mg/mL at 4 hours in FaSSIF media.
 42. The citrate salt of claim 23, wherein the Type A has a water sorption of 6% at 25° C. and 80% relative humidity as determined by DVS.
 43. A pharmaceutical composition comprising the pharmaceutically acceptable salt of claim 1 and a pharmaceutically acceptable carrier.
 44. A method of preparing a pharmaceutical composition for injection, comprising dissolving the pharmaceutically acceptable salt of claim 1 in a pharmaceutically acceptable carrier.
 45. The method of claim 44, wherein the pharmaceutically acceptable salt is dissolved in the pharmaceutically acceptable carrier within 24 hours.
 46. A pharmaceutical composition made by dissolving the pharmaceutically acceptable salt of claim 1 in a pharmaceutically acceptable carrier.
 47. The pharmaceutical composition of claim 46, wherein the pharmaceutical composition is suitable for injection into a human.
 48. The pharmaceutical composition of claim 46, wherein the pharmaceutical composition is suitable for intravenous injection into a human.
 49. A method of treating a fungal infection in a patient in need thereof, comprising administering to the patient a pharmaceutical composition comprising an effective amount of the pharmaceutically acceptable salt of claim
 1. 50. The method of claim 49, wherein the pharmaceutical composition is suitable for injection into a human.
 51. The method of claim 49, wherein the pharmaceutical composition is suitable for intravenous injection into a human.
 52. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of at least 2 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 53. The salt of claim 52, wherein the kinetic solubility is at least 4 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 54. The salt of claim 52, wherein the kinetic solubility is at least 8 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 55. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of from 2 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 56. The salt of claim 55, wherein the kinetic solubility is from 4 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 57. The salt of claim 55, wherein the kinetic solubility is from 8 mg/mL to 9 mg/mL at 24 hours in dextrose buffer at pH 5.5.
 58. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of at least 2 mg/mL at 4 hours in dextrose buffer at pH 5.5.
 59. The salt of claim 58, wherein the kinetic solubility is at least 4 mg/mL at 4 hours in dextrose buffer at pH 5.5.
 60. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of from 2 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5.
 61. The salt of claim 60, wherein the kinetic solubility is from 4 mg/mL to 5 mg/mL at 4 hours in dextrose buffer at pH 5.5.
 62. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of at least 2 mg/mL at 4 hours in phosphate buffer at pH 6.0.
 63. The salt of claim 62, wherein the kinetic solubility is at least 4 mg/mL at 4 hours in phosphate buffer at pH 6.0.
 64. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of from 2 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0.
 65. The salt of claim 64, wherein the kinetic solubility is from 4 mg/mL to 5 mg/mL at 4 hours in phosphate buffer at pH 6.0.
 66. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of at least 4.5 mg/mL at 24 hours in phosphate buffer at pH 6.0.
 67. The salt of claim 66, wherein the kinetic solubility is at least 7 mg/mL at 24 hours in phosphate buffer at pH 6.0.
 68. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of from 4.5 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.
 69. The salt of claim 68, wherein the kinetic solubility is from 7 mg/mL to 8 mg/mL at 24 hours in phosphate buffer at pH 6.0.
 70. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of 16 mg/mL at 1 hour in SGF media.
 71. The salt of claim 70, wherein the kinetic solubility is at least 17 mg/mL at 1 hour in SGF media.
 72. The salt of claim 70, wherein the kinetic solubility is at least 18 mg/mL at 1 hour in SGF media.
 73. The salt of claim 70, wherein the kinetic solubility is at least 20 mg/mL at 1 hour in SGF media.
 74. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of from 17 mg/mL to 21 mg/mL at 1 hour in SGF media.
 75. The salt of claim 74, wherein the kinetic solubility is from 18 mg/mL to 21 mg/mL at 1 hour in SGF media.
 76. The salt of claim 74, wherein the kinetic solubility is from 20 mg/mL to 21 mg/mL at 1 hour in SGF media.
 77. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of at least 17 mg/mL at 24 hours in FaSSIF media.
 78. The salt of claim 77, wherein the kinetic solubility is at least 22 mg/mL at 24 hours in FaSSIF media.
 79. A pharmaceutically acceptable salt of compound 1:

having a kinetic solubility of from 17 mg/mL to 22 mg/mL at 24 hours in FaSSIF media.
 80. The salt of claim 79, wherein the kinetic solubility of from 21 mg/mL to 22 mg/mL at 24 hours in FaSSIF media.
 81. A pharmaceutically acceptable salt according to any of claims 52, 55, 58, 60, 62, 64, 66, 68, 70, 74, 77, and 79 having a water sorption of not greater than 7% at 25° C. and 80% relative humidity as determined by DVS.
 82. A pharmaceutically acceptable salt according to claim 81 having a water sorption of from 2% to 7% at 25° C. and 80% relative humidity as determined by DVS.
 83. The salt of claim 82, wherein the water sorption is from 3% to 7% at 25° C. and 80% relative humidity as determined by DVS.
 84. The salt of claim 82, wherein the water sorption is from 6% to 7% at 25° C. and 80% relative humidity as determined by DVS. 